lncRNA SNHG6 functions as a ceRNA to up-regulate c-Myc expression via sponging let-7c-5p in hepatocellular carcinoma
| Autor: | Xiarong Hu, Chuping Xie, Siyuan Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Carcinoma Hepatocellular Biophysics Kaplan-Meier Estimate Biology Biochemistry Proto-Oncogene Proteins c-myc 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Humans Gene silencing Molecular Biology Gene Loss function Cell Proliferation Competing endogenous RNA Cell growth Gene Expression Profiling Liver Neoplasms Cell Biology Prognosis medicine.disease digestive system diseases Long non-coding RNA Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Hepatocellular carcinoma Correlation analysis Cancer research RNA RNA Long Noncoding |
| Zdroj: | Biochemical and Biophysical Research Communications. 519:901-908 |
| ISSN: | 0006-291X |
| Popis: | Emerging evidence has revealed that dysregulation of lncRNAs correlate with the development and progression of hepatocellular carcinoma (HCC). In the present study, we globally investigated the expression of SNHG6 in 31 cancer type, and we found that SNHG6 was highly expressed in various cancers, especially in HCC. High expression of SNHG6 was associated with progression and poor prognosis in patients with HCC. Gain of function and loss of function assays showed that SNHG6 promoted HCC cell proliferation. Gene Set Enrichment Analysis (GSEA) and correlation analysis suggested that SNHG6 positively correlated with c-Myc and its downstream targets. Ectopic overexpression of SNHG6 markedly increased the expression of c-Myc and its downstream targets, whereas silencing SNHG6 had the opposite effect on the expression of c-Myc and its downstream targets. Mechanistic assays revealed that SNHG6 acted as a competing endogenous RNA (ceRNA) to sponge let-7c-5p and thereby modulating the depression of c-Myc by let-7c-5p. Taken together, SNHG6 promotes HCC cell proliferation via competitively binding let-7c-5p in hepatocellular carcinoma. |
| Databáze: | OpenAIRE |
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