What determines host specificity in hyperspecialized plant parasitic nematodes?

Autor: Etienne Lord, Michael Sabeh, Benjamin Mimee, Eric Grenier, Marc St-Arnaud
Přispěvatelé: Agriculture and Agri-Food Canada (AAFC), Université de Montréal (UdeM), Jardin Botanique de Montréal, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), 2015-69004-23634, National Institute of Food and Agriculture, n.a., Agriculture and Agri-Food Canada, Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Agriculture and Agri-Food [Ottawa] (AAFC), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Agriculture and Agri-Food (AAFC), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
effecteur
RNA-Seq
Polymerase Chain Reaction
01 natural sciences
Transcriptome
Transcriptomic study
ComputingMilieux_MISCELLANEOUS
Regulator gene
nématode à kyste
2. Zero hunger
Genetics
0303 health sciences
gamme d'hôtes
Effector
globodera
effector
Plant-parasitic nematode
Research Article
Biotechnology
lcsh:QH426-470
Globodera rostochiensis
lcsh:Biotechnology
hosts range
Virulence
Biology
Host Specificity
03 medical and health sciences
lcsh:TP248.13-248.65
Invertebrate Zoology
Effectors
Globodera
Host range
Pathogenicity
RNA-seq
Animals
Tylenchoidea
Gene
Solanum tuberosum
030304 developmental biology
Sequence Analysis
RNA
Host (biology)
Gene Expression Profiling
Genetic Variation
biology.organism_classification
Zoologie des invertébrés
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
lcsh:Genetics
010606 plant biology & botany
Zdroj: BMC Genomics 1 (20), . (2019)
BMC Genomics
BMC Genomics, BioMed Central, 2019, 20 (1), pp.457. ⟨10.1186/s12864-019-5853-4⟩
BMC Genomics, 2019, 20 (1), pp.457. ⟨10.1186/s12864-019-5853-4⟩
BMC Genomics, Vol 20, Iss 1, Pp 1-13 (2019)
ISSN: 1471-2164
DOI: 10.1186/s12864-019-5853-4⟩
Popis: Background In hyperspecialized parasites, the ability to grow on a particular host relies on specific virulence factors called effectors. These excreted proteins are involved in the molecular mechanisms of parasitism and distinguish virulent pathogens from non-virulent related species. The potato cyst nematodes (PCN) Globodera rostochiensis and G. pallida are major plant-parasitic nematodes developing on numerous solanaceous species including potato. Their close relatives, G. tabacum and G. mexicana are stimulated by potato root diffusate but unable to establish a feeding site on this plant host. Results RNA sequencing was used to characterize transcriptomic differences among these four Globodera species and to identify genes associated with host specificity. We identified seven transcripts that were unique to PCN species, including a protein involved in ubiquitination. We also found 545 genes that were differentially expressed between PCN and non-PCN species, including 78 genes coding for effector proteins, which represent more than a 6-fold enrichment compared to the whole transcriptome. Gene polymorphism analysis identified 359 homozygous non-synonymous variants showing a strong evidence for selection in PCN species. Conclusions Overall, we demonstrated that the determinant of host specificity resides in the regulation of essential effector gene expression that could be under the control of a single or of very few regulatory genes. Such genes are therefore promising targets for the development of novel and more sustainable resistances against potato cyst nematodes. Electronic supplementary material The online version of this article (10.1186/s12864-019-5853-4) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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