A lectin fraction from green seaweed Caulerpa cupressoides inhibits inflammatory nociception in the temporomandibular joint of rats dependent from peripheral mechanisms
| Autor: | Hellíada Vasconcelos Chaves, Renata Line da Conceição Rivanor, Felipe Dantas Silveira, Natássia Albuquerque Ribeiro, Norma Maria Barros Benevides, Ellen Lima de Assis, Mirna Marques Bezerra, Danielle Rocha do Val, Lorena Vasconcelos Vieira, Álvaro Xavier Franco, Ismael Nilo Lino de Queiroz |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Nociception 0301 basic medicine Orofacial pain medicine.medical_treatment Interleukin-1beta Motor Activity Pharmacology Biochemistry 03 medical and health sciences 0302 clinical medicine stomatognathic system Structural Biology medicine Animals Caulerpa Rats Wistar Molecular Biology Saline Inflammation Analgesics Temporomandibular Joint biology Tumor Necrosis Factor-alpha business.industry Lectin General Medicine Rats Caulerpa cupressoides Temporomandibular joint Peripheral 030104 developmental biology medicine.anatomical_structure Cyclooxygenase 2 biology.protein Plant Lectins medicine.symptom business Cell Adhesion Molecules 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Biological Macromolecules. 115:331-340 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.04.065 |
| Popis: | Temporomandibular disorders are the second most common cause of orofacial pain mediated by inflammatory compounds, which in many cases leads to chronic orofacial pain. This study assessed the antinociceptive and anti-inflammatory effects of a lectin from the green seaweed Caulerpa cupressoides (CcL) on hypernociception inflammatory in TMJ of rats and investigated the involvement of different mechanisms. Rats received i.v. CcL 30 min prior to injection of flogistic agentes or 0.9% saline into the left TMJ. Pretreatment with CcL (0. 1; 1 or 10 mg/kg) promoted a reduction (p 0.05) of inflammatory hypernociception induced by 1.5% Formalin along with inhibition of inflammatory plasma extravasation, cytokines levels, ciclooxigenase-2, and intercellular adhesion molecule (ICAM-1). CcL was able to inhibit the nociceptive response induced by 1.5% Capsaicin, suggesting that CcL has an antinociceptive effect, acting directly on the primary nociceptive neurons. CcL also inhibited the nociceptive response induced by Carrageenan (100 μg/TMJ) or Serotonin (5-HT) (225 μg/TMJ). In conclusion, the results demonstrate that administration of CcL has a potential antinociceptive and anti-inflammatory effect, with a mechanism that is partially dependent on TNF-α, IL-1β, COX-2 and ICAM-1 inhibition and independently from the cannabinoide and opioid system and NO/cGMP/PKG/K |
| Databáze: | OpenAIRE |
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