Changes in Oleic Acid Content of Transgenic Soybeans by Antisense RNA Mediated Posttranscriptional Gene Silencing
| Autor: | Li Haiyun, Yang Jing, Dongquan Guo, Yingshan Dong, Yao Yao, Yuanyu Zhang, Wen-jing Xu, Xiangdong Yang, Guang-xun Qi, Ling Zhang, Qiyun Li, Xing Guojie |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
lcsh:QH426-470 Article Subject Linoleic acid Transgene Pharmaceutical Science Fatty acid food and beverages Biology Biochemistry Antisense RNA Palmitic acid lcsh:Genetics chemistry.chemical_compound Oleic acid chemistry Complementary DNA Genetics Molecular Biology Gene Research Article |
| Zdroj: | International Journal of Genomics International Journal of Genomics, Vol 2014 (2014) |
| ISSN: | 2314-436X |
| DOI: | 10.1155/2014/921950 |
| Popis: | The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturaseGmFad2-1bgene by using antisense RNA in soybean Williams 82. By employing the soybean cotyledonary-node method, a part of the cDNA of soybeanGmFad2-1b801 bp was cloned for the construction of a pCAMBIA3300 vector under the soybean seed promoterBCSP. Leaf painting, LibertyLink strip, PCR, Southern blot, qRT-PCR, and fatty acid analysis were used to detect the insertion and expression ofGmFad2-1bin the transgenic soybean lines. The results indicate that the metabolically engineered plants exhibited a significant increase in oleic acid (up to 51.71%) and a reduction in palmitic acid (to |
| Databáze: | OpenAIRE |
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