Cytotoxicity of Titanate-Calcium Complexes to MC3T3 Osteoblast-Like Cells
| Autor: | Jeanie L. Drury, Yen-Wei Chen, Kathryn M. L. Taylor-Pashow, Joelle Moussi, David T. Hobbs, John C. Wataha |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Pathology
medicine.medical_specialty Article Subject lcsh:Medicine chemistry.chemical_element 02 engineering and technology Calcium Biology General Biochemistry Genetics and Molecular Biology Cell Line Metal 03 medical and health sciences Mice 0302 clinical medicine stomatognathic system Osteogenesis medicine Cytotoxic T cell Animals MC3T3 Cytotoxicity Titanium Osteoblasts General Immunology and Microbiology lcsh:R Osteoblast Cell Differentiation 030206 dentistry General Medicine Metabolism 021001 nanoscience & nanotechnology medicine.anatomical_structure chemistry Biochemistry visual_art Toxicity visual_art.visual_art_medium 0210 nano-technology human activities Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2016 (2016) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Monosodium titanates (MST) are a relatively novel form of particulate titanium dioxide that have been proposed for biological use as metal sorbents or delivery agents, most recently calcium (II). In these roles, the toxicity of the titanate or its metal complex is crucial to its biological utility. The aim of this study was to determine the cytotoxicity of MST and MST-calcium complexes with MC3T3 osteoblast-like cells; MST-Ca(II) complexes could be useful to promote bone formation in various hard tissue applications. MC3T3 cells were exposed to native MST or MST-Ca(II) complexes for 24–72 h. A CellTiter-Blue® assay was employed to assess the metabolic activity of the cells. The results showed that MST and MST-Ca(II) suppressed MC3T3 metabolic activity significantly in a dose-, time-, and cell-density-dependent fashion. MST-Ca(II) suppressed MC3T3 metabolism in a statistically identical manner as native MST at all concentrations. We concluded that MST and MST-Ca(II) are significantly cytotoxic to MC3T3 cells through a mechanism yet unknown; this is a potential problem to the biological utility of these complexes. |
| Databáze: | OpenAIRE |
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