Tannic acid elicits selective antitumoral activity in vitro and inhibits cancer cell growth in a preclinical model of glioblastoma multiforme
| Autor: | Luiza Spohr, Fernanda G. Sekine, Francine Hehn de Oliveira, Bernardo de Moraes Meine, Nicolly Espindola Gelsleichter, Mayara Sandrielly Pereira Soares, Nathalia Stark Pedra, Juliana Hofstatter Azambuja, Francieli Moro Stefanello, Natália Pontes Bona, Roselia Maria Spanevello, Elizandra Braganhol, Lorenço Torres Mendonça, Elita Ferreira da Silveira |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Programmed cell death Cell cycle checkpoint Cell Survival Antineoplastic Agents Biochemistry 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine In vivo Cell Line Tumor Animals Rats Wistar Cytotoxicity Cell adhesion Cell Proliferation Dose-Response Relationship Drug Chemistry Brain Neoplasms Cell cycle Xenograft Model Antitumor Assays Rats 030104 developmental biology Apoptosis Cancer cell Cancer research Female Neurology (clinical) Glioblastoma Tannins 030217 neurology & neurosurgery |
| Zdroj: | Metabolic brain disease. 35(2) |
| ISSN: | 1573-7365 |
| Popis: | Glioblastoma is a devastating tumor affecting the central nervous system with infiltrative capacity, high proliferation rate and chemoresistance. Therefore, it is urgent to find new therapeutic alternatives that improve this prognosis. Herein, we focused on tannic acid (TA) a polyphenol with antioxidant and antiproliferative activities. In this work, the antitumor and antioxidant effects of TA on rat (C6) glioblastoma cells and their cytotoxicity relative to primary astrocyte cultures were evaluated in vitro. Cells were exposed to TA of 6.25 to 75 μM for 24, 48 and/or 72 h. In addition, colony formation, migration and cell adhesion were analyzed and flow cytometry was used to analyze cell death and cell cycle. Next, the action of TA was evaluated in a preclinical glioblastoma model performed on Wistar rats. In this protocol, the animals were treated with a dose of 50 mg/kg/day TA for 15 days. Our results demonstrated that TA induced in vitro selective antiglioma activity, not demonstrating cytotoxicity in astrocyte culture. It induced cell death by apoptosis and cell cycle arrest, reducing formation and size of colonies, cell migration/adhesion and showing to be a potential antioxidant. Interestingly, the antiglioma effect was also observed in vivo, as TA decreased tumor volume by 55%, accompanied by an increase in the area of intratumoral necrosis and infiltration of lymphocytes without causing systemic damage. To the best of our knowledge, this is the first study to report TA activity in a GBM preclinical model. Thus, this natural compound is promising as a treatment for glioblastoma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink