Influence of liver hepatitis C virus RNA and hepatitis C virus genotype on Fas-mediated apoptosis after liver transplantation for hepatitis C
| Autor: | Brenot C, Didier Samuel, H. Bismuth, Di Martino, Cyrille Feray, F Saurini, Michel Reynes, Paradis |
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| Předmět: |
Male
Cirrhosis Genotype Hepatitis C virus medicine.medical_treatment Hepacivirus Apoptosis DNA Fragmentation Liver transplantation medicine.disease_cause Liver disease medicine Humans fas Receptor Hepatitis Transplantation biology virus diseases Hepatitis C Viral Load medicine.disease biology.organism_classification Virology digestive system diseases Liver Immunology RNA Viral Female Follow-Up Studies |
| Zdroj: | Scopus-Elsevier |
| Popis: | Background. Recurrent hepatitis C virus (HCV) infection after liver transplantation is characterized by a high level of intrahepatic HCV replication and more severe liver damage in case of genotype 1b infection. We investigated the involvement of apoptosis in recurrent HCV liver disease, and its possible links with histological findings, HCV genotype, liver HCV RNA level, and liver Fas mRNA level. Methods. We studied 61 liver graft biopsy specimens from 25 patients transplanted for HCV-related cirrhosis. DNA fragmentation was determined semi-quantitatively by in situ end labeling. HCV RNA and liver Fas mRNA were determined in parallel by quantitative polymerase chain reaction, with ribosomal 28S RNA as internal standard. Results. Apoptotic lesions were predominantly portal (nonhepatocytic) or lobular (hepatocytic). Both were correlated with serum aminotransferase levels. The degree of portal apoptosis correlated with acute rejection (P |
| Databáze: | OpenAIRE |
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