Expression of multidrug resistance-associated proteins in rhabdomyosarcomas before and after chemotherapy

Autor: E.J.S.M. de Bont, E. van den Berg, Rudy Komdeur, J.W. Klunder, W.M. Molenaar, Harald J. Hoekstra, W.T.A. van der Graaf
Přispěvatelé: Damage and Repair in Cancer Development and Cancer Treatment (DARE), Stem Cell Aging Leukemia and Lymphoma (SALL)
Jazyk: angličtina
Rok vydání: 2003
Předmět:
Adult
Male
Pathology
medicine.medical_specialty
Adolescent
multidrug resistance-associated proteins
Cellular differentiation
LINE
ACUTE MYELOID-LEUKEMIA
medicine.disease_cause
TREATMENT LEADS
THERAPEUTIC DIFFERENTIATION
Pathology and Forensic Medicine
Desmin
CARCINOMA SW-620 CELLS
medicine
Humans
ATP Binding Cassette Transporter
Subfamily B
Member 1
Rhabdomyosarcoma
Child
P-glycoprotein
Vault Ribonucleoprotein Particles
DRUG-RESISTANCE
biology
CHILDHOOD RHABDOMYOSARCOMAS
Infant
P-GLYCOPROTEIN
Cell Differentiation
differentiation
medicine.disease
GENE
Neoplasm Proteins
Multiple drug resistance
lung resistance-related protein
biology.protein
ACTINOMYCIN-D
Female
lipids (amino acids
peptides
and proteins)
Sarcoma
Multidrug Resistance-Associated Protein 1
rhabdomyosarcoma
Neoplasm Recurrence
Local
Carcinogenesis
Multidrug Resistance-Associated Proteins
Zdroj: Human Pathology, 34(2), 150-155. W B SAUNDERS CO-ELSEVIER INC
ISSN: 0046-8177
Popis: Rhabdomyosarcomas generally respond well to chemotherapy, and the residual lesions often are better differentiated than their primaries. This phenomenon may be explained by selective multidrug resistance (MDR) of differentiated tumor cell populations. We assess the role of MDR proteins in chemotherapy-induced differentiation in rhabdomyosarcomas in a clinical setting. Paraffin-embedded samples of 13 pairs of primary untreated rhabdomyosarcomas and their residual, recurrent, or metastatic lesions after chemotherapy were assessed for expression of MDR proteins, including P-glycoprotein (Pgp), multidrug resistance-associated protein (MRP-1), and lung resistance-related protein (LRP). Expression was semiquantitatively scored based on the percentage of isolated immunoreactive tumor cells as follows: 0, negative; 0.5, 75%. All specimens after chemotherapy, except the late recurrences, were better differentiated than their primary, untreated specimens. Pgp or MRP-1 expression did not change significantly, but LRP expression increased significantly after chemotherapy. In both untreated and treated samples, LRP was expressed primarily in differentiated cells. The findings indicate that the in vivo expression of LRP, but not of Pgp and MRP-1, is induced by chemotherapeutic treatment in rhabdomyosarcomas. The preferential expression of LRP in differentiated cells and the subsequent more extensive expression after chemotherapy suggests that LRP plays a role in therapy-induced differentiation. Copyright 2003, Elsevier Science (USA). All rights reserved.
Databáze: OpenAIRE
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