Differential usage of COX-1 and COX-2 in prostaglandin production by mast cells and basophils
| Autor: | Yoshinori Yamanishi, Masashi Minami, Tomoyuki Bando, Naoko Nagano, Hajime Karasuyama, Setsuko Fujita, Soichiro Yoshikawa |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell type LTB4 leukotriene B4 Biophysics Stimulation chemical and pharmacologic phenomena Immunoglobulin E Biochemistry lcsh:Biochemistry 03 medical and health sciences chemistry.chemical_compound Immune system LTA4 leukotriene A4 HETE hydroxyeicosatetraenoic acid parasitic diseases PGE2 prostaglandin E2 lcsh:QD415-436 Celecoxib (PubChem CID: 2662) LC-MS/MS lcsh:QH301-705.5 LOX lipoxygenase BW-A4C (PubChem CID: 6438354) biology BMBAs bone marrow derived basophils OVA Ovalbumin Chemistry Effector hemic and immune systems Lipid signaling LTD4 leukotriene D4 SC-560 (PubChem CID: 4306515) COX-2 Basophils Interleukin 33 COX cyclooxygenase 030104 developmental biology lcsh:Biology (General) TNP 2 4 6-trinitrophenyl Immunology biology.protein PGD2 prostaglandin D2 Mast cells Prostaglandins Eicosanoids LTC4 leukotriene C4 Histamine Research Article BMMCs bone marrow derived mast cells |
| Zdroj: | Biochemistry and Biophysics Reports, Vol 10, Iss C, Pp 82-87 (2017) Biochemistry and Biophysics Reports |
| ISSN: | 2405-5808 |
| DOI: | 10.1016/j.bbrep.2017.03.004 |
| Popis: | Basophils have been erroneously considered as minor relatives of mast cells, due to some phenotypic similarity between them. While recent studies have revealed non-redundant roles for basophils in various immune responses, basophil-derived effector molecules, including lipid mediators, remain poorly characterized, compared to mast cell-derived ones. Here we analyzed and compared eicosanoids produced by mouse basophils and mast cells when stimulated with IgE plus allergens. The production of 5-LOX metabolites such as LTB4 and 5-HETE was detected as early as 0.5 h post-stimulation in both cell types, even though their amounts were much smaller in basophils than in mast cells. In contrast, basophils and mast cells showed distinct time course in the production of COX metabolites, including PGD2, PGE2 and 11-HETE. Their production by mast cells was detected at both 0.5 and 6 h post-stimulation while that by basophils was detectable only at 6 h. Of note, mast cells showed 8–9 times higher levels of COX-1 than did basophils at the resting status. In contrast to unaltered COX-1 expression with or without stimulation, COX-2 expression was up-regulated in both cell types upon activation. Importantly, when activated, basophils expressed 4–5 times higher levels of COX-2 than did mast cells. In accordance with these findings, the late-phase production of the COX metabolites by basophils was completely ablated by COX-2 inhibitor whereas the early-phase production by mast cells was blocked by COX-1 but not COX-2 inhibitor. Thus, the production of COX metabolites is differentially regulated by COX-1 and COX-2 in basophils and mast cells. Highlights • Basophils and mast cells show distinct time course of COX metabolite production. • Basophils and mast cells show differential expression and induction of COX isoforms. • COX metabolite production by basophils but not mast cells is mediated by COX-2. |
| Databáze: | OpenAIRE |
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