Application of Immunoprofiling Using Multiplexed Immunofluorescence Staining Identifies the Prognosis of Patients with High-Grade Serous Ovarian Cancer
Autor: | Young Jae Lee, Chang Ohk Sung, Ha Young Lee, Min-Je Kim, Yong-Man Kim, Sung Wan Kang, Shin-Wha Lee |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Oncology
medicine.medical_treatment Fluorescent Antibody Technique B7-H1 Antigen Medicine Stage (cooking) Biology (General) Spectroscopy Ovarian Neoplasms CD20 biology quantitative analysis musculoskeletal neural and ocular physiology FOXP3 Forkhead Transcription Factors General Medicine Middle Aged Prognosis Computer Science Applications Chemistry ovarian cancer Immunohistochemistry Female Adult PD-L1 medicine.medical_specialty QH301-705.5 CD8 Antigens macromolecular substances Article Catalysis Inorganic Chemistry Internal medicine FoxP3 multiplex IHC Biomarkers Tumor immunoprofiling Humans Physical and Theoretical Chemistry Molecular Biology QD1-999 Aged Chemotherapy Staining and Labeling business.industry Organic Chemistry CD8 medicine.disease Survival Analysis Cystadenocarcinoma Serous nervous system Multivariate Analysis biology.protein Neoplasm Grading business Ovarian cancer |
Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 17 International Journal of Molecular Sciences, Vol 22, Iss 9638, p 9638 (2021) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms22179638 |
Popis: | Immunoprofiling has an established impact on the prognosis of several cancers however, its role and definition in high-grade serous ovarian cancer (HGSOC) are mostly unknown. This study is to investigate immunoprofiling which could be a prognostic factor in HGSOC. We produced tumor microarrays of 187 patients diagnosed with HGSOC. We performed a multiplexed immunofluorescence staining using Opal Multiplex IHC kit and quantitative analysis with Vectra-Inform system. The expression intensities of programmed death-ligand 1 (PD-L1), CD4, CD8, CD20, FoxP3, and CK in whole tumor tissues were evaluated. The enrolled patients showed general characteristics, mostly FIGO stage III/IV and responsive to chemotherapy. Each immune marker showed diverse positive densities, and each tumor sample represented its immune characteristics as an inflamed tumor or noninflamed tumor. No marker was associated with survival as a single one. Interestingly, high ratios of CD8 to FoxP3 and CD8 to PD-L1 were related to the favorable overall survival (77 vs. 39 months, 84 vs. 47 months, respectively), and CD8 to PD-L1 ratio was also a significant prognostic factor (HR 0.621, 95% CI 0.420–0.917, p = 0.017) along with well-known clinical prognostic factors. Additionally, CD8 to PD-L1 ratio was found to be higher in the chemosensitive group (p = 0.034). In conclusion, the relative expression levels of CD8, FoxP3, and PD-L1 were significantly related to the clinical outcome of patients with HGSOC, which could be a kind of significant immunoprofiling of ovarian cancer patients to apply for treatment. |
Databáze: | OpenAIRE |
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