Application of Immunoprofiling Using Multiplexed Immunofluorescence Staining Identifies the Prognosis of Patients with High-Grade Serous Ovarian Cancer

Autor: Young Jae Lee, Chang Ohk Sung, Ha Young Lee, Min-Je Kim, Yong-Man Kim, Sung Wan Kang, Shin-Wha Lee
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Oncology
medicine.medical_treatment
Fluorescent Antibody Technique
B7-H1 Antigen
Medicine
Stage (cooking)
Biology (General)
Spectroscopy
Ovarian Neoplasms
CD20
biology
quantitative analysis
musculoskeletal
neural
and ocular physiology
FOXP3
Forkhead Transcription Factors
General Medicine
Middle Aged
Prognosis
Computer Science Applications
Chemistry
ovarian cancer
Immunohistochemistry
Female
Adult
PD-L1
medicine.medical_specialty
QH301-705.5
CD8 Antigens
macromolecular substances
Article
Catalysis
Inorganic Chemistry
Internal medicine
FoxP3
multiplex IHC
Biomarkers
Tumor
immunoprofiling
Humans
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
Aged
Chemotherapy
Staining and Labeling
business.industry
Organic Chemistry
CD8
medicine.disease
Survival Analysis
Cystadenocarcinoma
Serous
nervous system
Multivariate Analysis
biology.protein
Neoplasm Grading
business
Ovarian cancer
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 17
International Journal of Molecular Sciences, Vol 22, Iss 9638, p 9638 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22179638
Popis: Immunoprofiling has an established impact on the prognosis of several cancers
however, its role and definition in high-grade serous ovarian cancer (HGSOC) are mostly unknown. This study is to investigate immunoprofiling which could be a prognostic factor in HGSOC. We produced tumor microarrays of 187 patients diagnosed with HGSOC. We performed a multiplexed immunofluorescence staining using Opal Multiplex IHC kit and quantitative analysis with Vectra-Inform system. The expression intensities of programmed death-ligand 1 (PD-L1), CD4, CD8, CD20, FoxP3, and CK in whole tumor tissues were evaluated. The enrolled patients showed general characteristics, mostly FIGO stage III/IV and responsive to chemotherapy. Each immune marker showed diverse positive densities, and each tumor sample represented its immune characteristics as an inflamed tumor or noninflamed tumor. No marker was associated with survival as a single one. Interestingly, high ratios of CD8 to FoxP3 and CD8 to PD-L1 were related to the favorable overall survival (77 vs. 39 months, 84 vs. 47 months, respectively), and CD8 to PD-L1 ratio was also a significant prognostic factor (HR 0.621, 95% CI 0.420–0.917, p = 0.017) along with well-known clinical prognostic factors. Additionally, CD8 to PD-L1 ratio was found to be higher in the chemosensitive group (p = 0.034). In conclusion, the relative expression levels of CD8, FoxP3, and PD-L1 were significantly related to the clinical outcome of patients with HGSOC, which could be a kind of significant immunoprofiling of ovarian cancer patients to apply for treatment.
Databáze: OpenAIRE
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