The MADS-Box Gene CsSHP Participates in Fruit Maturation and Floral Organ Development in Cucumber
| Autor: | Ran Gu, Weiyuan Song, Gen Che, Xiaolan Zhang, Zhongyi Wang, Zhihua Cheng, Deguo Han, Xiaofeng Liu, Shibin Zhuo, Junjun Shen, Zhaoyang Zhou |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Stamen fruit maturation Plant Science lcsh:Plant culture 01 natural sciences abscisic acid Cucumis sativus SHATTERPROOF 03 medical and health sciences chemistry.chemical_compound Arabidopsis lcsh:SB1-1110 Abscisic acid MADS-box floral organ identity biology food and beverages Ripening biology.organism_classification Horticulture 030104 developmental biology chemistry Ectopic expression Silique cucumber Cucumis 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 10 (2020) |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2019.01781 |
| Popis: | Cucumber is an important vegetable crop bearing fleshy pepo fruit harvested immature. Fruits left unpicked in time during summer production, as well as unfavorable environmental conditions during post-harvest shelf, will cause cucumber fruits to turn yellow and ripen, and thus impair the market value. Identification of maturity-related genes is of great agricultural and economic importance for cucumber production. Here, we isolated and characterized a MADS-box gene, Cucumis sativus SHATTERPROOF (CsSHP) in cucumber. Expression analysis indicated that CsSHP was specifically enriched in reproductive organs including stamens and carpels. Ectopic expression of CsSHP was unable to rescue the indehiscence silique phenotype of shp1 shp2 mutant plant in Arabidopsis. Instead, overexpression of CsSHP resulted in early flowering, precocious phenotypes, and capelloid organs in wild-type Arabidopsis. Biochemical analysis indicated that CsSHP directly interacted with cucumber SEPALLATA (SEP) proteins. CsSHP expression increased significantly during the yellowing stage of cucumber ripening, and was induced by exogenous application of abscisic acid (ABA). Therefore, CsSHP may participate in fruit maturation through the ABA pathway and floral organ specification via interaction with CsSEPs to form protein complex in cucumber. |
| Databáze: | OpenAIRE |
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