NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes
| Autor: | Yiping Tu, Ken Matsumoto, Patrick J. Heizer, Haibo Jiang, Holger Gerhardt, Peter Tontonoz, Xuchen Hu, Rachel S. Jung, Rochelle M Ellison, Thomas A. Weston, Loren G. Fong, Cuiwen He, Stephen G. Young |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gastric gavage medicine.medical_specialty Brown Adipocytes Lipoproteins Biophysics Spectrometry Mass Secondary Ion Adipose tissue White adipose tissue Biochemistry Article 03 medical and health sciences 13c enrichment Nutrient Internal medicine Brown adipose tissue medicine Animals Molecular Biology Receptors Lipoprotein Carbon Isotopes Chemistry Metabolic heterogeneity Fatty Acids Nutrients Cell Biology Lipids Mice Mutant Strains Mice Inbred C57BL Adipocytes Brown Glucose 030104 developmental biology Endocrinology medicine.anatomical_structure |
| Zdroj: | Biochemical and Biophysical Research Communications. 504:899-902 |
| ISSN: | 0006-291X |
| Popis: | Heterogeneity in the metabolic properties of adipocytes in white adipose tissue has been well documented. We sought to investigate metabolic heterogeneity in adipocytes of brown adipose tissue (BAT), focusing on heterogeneity in nutrient uptake. To explore the possibility of metabolic heterogeneity in brown adipocytes, we used nanoscale secondary ion mass spectrometry (NanoSIMS) to quantify uptake of lipids in adipocytes interscapular BAT and perivascular adipose tissue (PVAT) after an intravenous injection of triglyceride-rich lipoproteins (TRLs) containing [(2)H]triglycerides ((2)H-TRLs). The uptake of deuterated lipids into brown adipocytes was quantified by NanoSIMS. We also examined (13)C enrichment in brown adipocytes after administering [(13)C]glucose or (13)C-labeled mixed fatty acids by gastric gavage. The uptake of (2)H-TRLs-derived lipids into brown adipocytes was heterogeneous, with (2)H enrichment in adjacent adipocytes varying by more than fourfold. We also observed substantial heterogeneity in (13)C enrichment in adjacent brown adipocytes after administering [(13)C]glucose or [(13)C]fatty acids by gastric gavage. The uptake of nutrients by adjacent brown adipocytes within a single depot is variable, suggesting that there is heterogeneity in the metabolic properties of brown adipocytes. |
| Databáze: | OpenAIRE |
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