Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp
| Autor: | Eris Risdiana, Bambang Hanggono, Kathy F.J. Tang, Luis Fernando Aranguren, Brenda White-Noble, Margeaux M. Schmidt, Jee Eun Han, Patharapol Piamsomboon |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Veterinary medicine animal structures Aquaculture Polymerase Chain Reaction Shrimp farming 03 medical and health sciences Feces Penaeidae parasitic diseases Parasite hosting Animals Penaeus Carapace Ecology Evolution Behavior and Systematics Shellfish biology fungi Midgut 04 agricultural and veterinary sciences Enterocytozoon biology.organism_classification Shrimp Fishery 030104 developmental biology 040102 fisheries 0401 agriculture forestry and fisheries Hepatopancreas |
| Zdroj: | Journal of invertebrate pathology. 140 |
| ISSN: | 1096-0805 |
| Popis: | White feces syndrome (WFS) is an emerging problem for penaeid shrimp farming industries in SE Asia countries, Thailand, Malaysia, Vietnam, Indonesia, China, and in India. This occurrence of this syndrome is usually first evidenced by the appearance of white fecal strings floating on surface of the shrimp ponds. The gross signs of affected shrimp include the appearance of a whitish hindgut and loose carapace, and it is associated with reduced feeding and growth retardation. To investigate the nature of the white feces syndrome, samples of white feces and shrimp hepatopancreas tissue were collected from Penaeus vannamei in affected farms in Indonesia, and these were examined histologically. Within the white feces, we found densely packed spores of the microsporidian Enterocytozoon hepatopenaei (abbreviated as EHP) and relatively fewer numbers of rod-shaped bacteria. From WFS ponds, hepatopancreas samples form 30 individual shrimp were analyzed by histology and in situ hybridization. The results showed that all of the shrimp examined were infected with EHP accompanied by septic hepatopancreatic necrosis (SHPN). Midgut epithelial cells were also infected and this increased the number of tissue types being affected by EHP. By PCR, EHP was detected in all the samples analyzed from WFS-affected ponds, but not in those sampled from healthy shrimp ponds. To determine the modes of transmission for this parasite, we performed feeding and cohabitation bioassays, the results showed that EHP can be transmitted through per os feeding of EHP-infected hepatopancreas tissue to healthy shrimp and through cohabitation ofinfected and healthy shrimp. In addition, we found the use of Fumagillin-B, an antimicrobial agent, was ineffective in either reducing or eliminating EHP in infected shrimp. |
| Databáze: | OpenAIRE |
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