Microfluidic-RT-LAMP chip for the point-of-care detection of emerging and re-emerging enteric coronaviruses in swine

Autor: Xueen Fang, Jingyun Ma, Liu Yanhong, Chen Yonghui, Ling Zhou, Li Qianniu, Lu Xiandong, Yuan Sun, Tian Lan, Mengkan Du
Rok vydání: 2020
Předmět:
Diarrhea
2019-20 coronavirus outbreak
Acute diarrhea
Coronavirus disease 2019 (COVID-19)
Swine
viruses
Point-of-Care Systems
Early detection
02 engineering and technology
01 natural sciences
Biochemistry
Sensitivity and Specificity
Article
Analytical Chemistry
Feces
Limit of Detection
Lab-On-A-Chip Devices
Environmental Chemistry
Emerging and re-emerging swine coronaviruses
Animals
Spectroscopy
Point of care
POC
biology
Chemistry
Porcine epidemic diarrhea virus
010401 analytical chemistry
Alphacoronavirus
Reproducibility of Results
021001 nanoscience & nanotechnology
biology.organism_classification
Virology
0104 chemical sciences
Porcine deltacoronavirus
Microfluidic chip
Coronavirus
Loop mediated isothermal amplification (LAMP)
RNA
Viral
Disease prevention
0210 nano-technology
Nucleic Acid Amplification Techniques
Zdroj: Analytica Chimica Acta
ISSN: 1873-4324
Popis: Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome-coronavirus (SADS-CoV) are three emerging and re-emerging coronaviruses (CoVs). Symptoms caused by these three viruses are extremely similar, including acute diarrhea, vomiting and even death in piglets. To date, strict biosecurity is still the most effective disease prevention and control measures, and the early detection of pathogens is the most important link. Here, we developed a microfluidic-RT-LAMP chip detection system for the first time, which could detected PEDV, PDCoV and SADS-CoV simultaneously, and had advantages of rapid, simple, sensitive, high-throughput, and accurate at point-of-care settings. The lowest detection limits of the microfluidic-RT-LAMP chip method are 101 copies/μL, 102 copies/μL and 102 copies/μL for PEDV, PDCoV and SADS-CoV, respectively. The whole detection procedure can be finished rapidly in 40 min without any cross-reaction with other common swine viruses. A total of 173 clinical swine fecal samples characterized with diarrheal symptoms were used to evaluate the performance of the newly developed system, which presented good stabilities (C.V.s
Graphical abstract Image 1
Highlights • In this research, we firstly integrated microfluidic chip with the RT-LAMP technology for the high-throughput and multi-target detection of three emerging and re-emerging swine coronaviruses. • The microfluidic-RT-LAMP chip system established greatly facilitated the rapid, specific and sensitive diagnosis of the PEDV, PDCoV and SADS-CoV. • The application of cost-effective microfluidic chip device is characterized with excellent prospects in swine enteropathogenic coronaviruses monitoring and control for remote livestock farms.
Databáze: OpenAIRE
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