Antiplasmodial and anti-inflammatory activities of Canthium henriquesianum (K. Schum), a plant used in traditional medicine in Burkina Faso
| Autor: | Paolo Coghi, Nicoletta Basilico, Enrica Bosisio, Jacques Simpore, Simplice D. Karou, Sarah D'Alessandro, Diego Monti, D. Ilboudo, Yolanda Corbett, Richard Sawadogo, Charlemagne Gnoula, Silvia Parapini, Mario Dell'Agli, Donatella Taramelli, Jean-Baptiste Nikiema |
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| Přispěvatelé: | Ilboudo, D, Basilico, N, Parapini, S, Corbett, Y, D'Alessandro, S, Dell'Agli, M, Coghi, P, Karou, S, Sawadogo, R, Gnoula, C, Simpore, J, Nikiema, J, Monti, D, Bosisio, E, Taramelli, D |
| Rok vydání: | 2013 |
| Předmět: |
Interleukin-1beta
Anti-Inflammatory Agents Antimalarial Rubiaceae Pharmacology Canthium chemistry.chemical_compound Chloroquine Drug Discovery Medicine African Traditional Cells Cultured Plant Stems biology Traditional medicine Hemozoin Anti-Inflammatory Agent Gardenia Chromone Fibroblast Antimalarial plant Plant Leave Human medicine.drug Cell Survival medicine.drug_class Plasmodium falciparum Plant Stem Anti-inflammatory Plant Extract Antimalarials Cell Line Tumor Burkina Faso parasitic diseases medicine Humans MTT assay Plant Extracts Tumor Necrosis Factor-alpha Drug Discovery3003 Pharmaceutical Science Fibroblasts biology.organism_classification Malaria Plant Leaves chemistry Antiplasmodial activity Vernonia |
| Zdroj: | Journal of Ethnopharmacology. 148:763-769 |
| ISSN: | 0378-8741 |
| Popis: | Ethnopharmacological relevance Canthium henriquesianum (K. Schum) is traditionally used in Burkina Faso for the treatment of malaria, but has not been properly investigated, yet. The aim of this study was to characterize in vitro the antiplasmodial and the anti-inflammatory activity of extracts from Canthium henriquesianum (K. Schum). In parallel, extracts of Gardenia sokotensis (Hutch) and Vernonia colorata (Willd), also traditionally used together in Burkina Faso and already reported with antimalarial activity, were compared. Materials and methods Plant extracts were tested in vitro for antimalarial activity against chloroquine susceptible (D10) and resistant (W2) strains of Plasmodium falciparum using the lactate dehydrogenase assay. Cell cytotoxicity was assessed on human dermal fibroblast (HDF) by the MTT assay. The selectivity index (SI) was used as the ratio of the activity against the parasites compared to the toxicity of the plant extract against HDF. In vitro cytokine production was assessed by ELISA technique. Results Canthium henriquesianum aqueous extract had a moderate antimalarial activity (IC507). Canthium henriquesianum diisopropyl ether extract was the most potent inhibitor of parasite growth with an IC 50 9.5 μg/ml on W2 and 8.8 μg/ml on D10 and limited toxicity (SI>2). Gardenia sokotensis and Vernonia colorata aqueous extracts were shown to be significantly less active (IC50≥50 μg/ml) with substantial toxicity. In addition, when the production of IL-1β and TNFα by lipopolysaccharide (LPS) or hemozoin (malaria pigment) stimulated human THP-1 monocytes was assayed, it was found that the extract of Canthium henriquesianum induced a dose-dependent inhibition of IL-1β, but not of TNFα production, thus confirming its traditional use as antipyretic. By NMR analysis, the chromone was identified as the mostly represented compound in the diisopropyl ether extract of Canthium henriquesianum. Chromone however, was less active as antimalarial than the crude extract and it did not inhibit cytokine production at not toxic doses, indicating that other molecules in the total extracts contribute to the antiplasmodial and anti-inflammatory activity. Conclusion Canthium henriquesianum seems to possess antimalarial activity in vitro and the ability to inhibit the production of the pyrogenic cytokine IL-1β. © 2013 Published by Elsevier Ireland Ltd |
| Databáze: | OpenAIRE |
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