pING Family of Conjugative Plasmids from the Extremely Thermophilic Archaeon Sulfolobus islandicus : Insights into Recombination and Conjugation in Crenarchaeota
| Autor: | Ingelore Holz, Roger A. Garrett, Wolfram Zillig, David Prangishvili, Harpreet Singh, Hien Phan, Qunxin She, Kenneth M. Stedman |
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| Rok vydání: | 2000 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Replication Origin Microbiology Genome DNA sequencing Sulfolobus chemistry.chemical_compound Plasmid Genome Archaeal ORFS Molecular Biology Recombination Genetic Genetics Base Sequence biology Temperature Crenarchaeota Genetic Variation Sequence Analysis DNA biology.organism_classification Open reading frame chemistry Conjugation Genetic DNA Transposable Elements Plasmids and Transposons DNA Plasmids |
| Zdroj: | Journal of Bacteriology. 182:7014-7020 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.182.24.7014-7020.2000 |
| Popis: | A novel family of conjugative plasmids from Sulfolobus comprising the active variants pING1, -4, and -6 and the functionally defective variants pING2 and -3, which require the help of an active variant for spreading, has been extensively characterized both functionally and molecularly. In view of the sparse similarity between bacterial and archaeal conjugation and the lack of a practical genetic system for Sulfolobus , we compared the functions and sequences of these variants and the previously described archaeal conjugative plasmid pNOB8 in order to identify open reading frames (ORFs) and DNA sequences that are involved in conjugative transfer and maintenance of these plasmids in Sulfolobus . The variants pING4 and -6 are reproducibly derived from pING1 in vivo by successive transpositions of an element from the Sulfolobus genome. The small defective but mobile variants pING2 and -3, which both lack a cluster of highly conserved ORFs probably involved in plasmid transfer, were shown to be formed in vivo by recombinative deletion of the larger part of the genomes of pING4 and pING6, respectively. The efficient occurrence of these recombination processes is further evidence for the striking plasticity of the Sulfolobus genome. |
| Databáze: | OpenAIRE |
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