UriSed 3 PRO automated microscope in screening bacteriuria at region-wide laboratory organization
| Autor: | Niina Tohmola, Tanja Holma, Anu Pätäri-Sampo, Vesa-Petteri Kouri, Sirpa Friman, Maaret Lehtonen, Anna Lempiäinen, Timo Kouri, Tero Pihlajamaa, Vesa Kirjavainen, Katariina Alagrund |
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| Přispěvatelé: | Medicum, HUSLAB, Department of Clinical Chemistry and Hematology, Department of Diagnostics and Therapeutics, Clinicum, HUS Internal Medicine and Rehabilitation |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Bacteriuria Phase contrast microscopy Clinical Biochemistry Urine Urinalysis Sensitivity and Specificity Biochemistry law.invention Automation 03 medical and health sciences 0302 clinical medicine law Internal medicine Humans Medicine UriSed 3 PRO Screening cultures Urine culture Urine particles Hungary Microscopy Urinary tract infection business.industry Biochemistry (medical) General Medicine medicine.disease Predictive value 030104 developmental biology 030220 oncology & carcinogenesis Urinary Tract Infections 3111 Biomedicine Laboratories business Bacteria screening Urine collection |
| Zdroj: | Clinica Chimica Acta. 516:149-156 |
| ISSN: | 0009-8981 |
| Popis: | Background and aims We assessed the possibility to rule out negative urine cultures by counting with UriSed 3 PRO (77 Elektronika, Hungary) at Helsinki and Uusimaa Hospital District. Materials and methods Bacteria counting of the UriSed 3 PRO automated microscope was verified with reference phase contrast microscopy against growth in culture. After acceptance into routine, results of bacteria and leukocyte counting from 56 426 specimens with eight UriSed 3 PRO instruments were compared against results from parallel samples cultured on chromogenic agar. Laboratory data including preanalytical details were accessed through the regional database of the Helsinki and Uusimaa Hospital District. Results A combined sensitivity of 87–92% and a negative predictive value of 90–96% with a specificity of 54–50% was reached, depending on criteria. Preanalytical data (incubation time in bladder) combined with the way of urine collection would improve these figures if reliable. Conclusions Complex patient populations, regional logistics and data interfases, and economics related to increased costs of additional particle counts against costs of screening cultures of all samples, did not support adaptation of a screening process of urine cultures. This conclusion was made locally, and may not be valid elsewhere. |
| Databáze: | OpenAIRE |
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