Curcumin represses the activity of inhibitor-κB kinase in dextran sulfate sodium-induced colitis by S-nitrosylation
| Autor: | Chien-Sheng Wu, Zwe-Ling Kong, Ning-Jo Kao, Jia-Yuan Hu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Curcumin Colon Immunology Anti-Inflammatory Agents Nitric Oxide Synthase Type II IκB kinase Pharmacology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot medicine Animals Immunology and Allergy Colitis Mice Inbred BALB C medicine.diagnostic_test Kinase Dextran Sulfate NF-kappa B NF-κB S-Nitrosylation NFKB1 medicine.disease I-kappa B Kinase 030104 developmental biology chemistry Biochemistry 030220 oncology & carcinogenesis Cytokines Nitrogen Oxides Inflammation Mediators Signal Transduction |
| Zdroj: | International Immunopharmacology. 38:1-7 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2016.05.015 |
| Popis: | In this study, we investigated the preventive effects of curcumin using dextran sulfate sodium (DSS)-induced colitis and the potential role of curcumin in regulation of anti-inflammation through S-nitrosylation. After curcumin treatment for 6days, the body weight and disease activity index of DSS-induced mice was alleviated and the colonic length was also rescued. Western blot presented that the protein expression of iNOS can be reduced by curcumin. Consistently, mRNA level of iNOS and pro-inflammatory cytokines, such as TNFα, IL-1β, and IL-6, was also repressed. Moreover, Curcumin reduced the amount of nitrite in DSS-induced colitis but not affected total S-nitrosylation level on proteins on day 6, indicating that curcumin inhibited NO oxidation. Furthermore, the protection of S-nitrosylation on IKKβ in DSS-induced colitis for 6days by curcumin caused the repression of IκB phosphorylation and NF-κB activation. In conclusion, this study verified that curcumin-mediated S-nitrosylation may be as an important regulator for anti-inflammation in DSS-induced colitis of mice. |
| Databáze: | OpenAIRE |
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