Effect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium -mediated transformation of tomato ( Lycopersicon esculentum Mill.)
| Autor: | H.-Q. Ling, M. W. Ganal, D. Kriseleit |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cefotaxime
Callus formation Agrobacterium fungi food and beverages Plant Science General Medicine Agrobacterium tumefaciens biochemical phenomena metabolism and nutrition Biology Carbenicillin biology.organism_classification Microbiology Callus Ticarcillin polycyclic compounds medicine bacteria Agronomy and Crop Science medicine.drug Transformation efficiency |
| Zdroj: | Plant Cell Reports. 17:843-847 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s002990050495 |
| Popis: | Ticarcillin/potassium clavulanate is a very effective combination of antibiotics to eliminate Agrobacterium tumefaciens during tomato transformation. It shows no toxicity to tomato tissues at a concentration of 150 mg/l and significantly promotes callus formation and shoot regeneration. The transformation frequency was raised more than 40% in comparison to cefotaxime. Cefotaxime itself did not inhibit callus growth in culture medium, but it clearly decreased shoot differentiation. Together with kanamycin, cefotaxime shows a strong negative effect on callus growth, shoot regeneration and transformation efficiency. Unlike the widely used carbenicillin and cefotaxime, ticarcillin/potassium clavulanate is light stable and resistant to inactivation by β-lactamase. Furthermore, ticarcillin/potassium clavulanate is more economical than carbenicillin and cefotaxime. In conclusion, ticarcillin/potassium clavulanate is a very good alternative to eliminate Agrobacterium tumefaciens in plant transformation and has the potential to be widely used for plants which are sensitive to carbenicillin and cefotaxime. |
| Databáze: | OpenAIRE |
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