Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1
| Autor: | Seicho Makihira, Keishi Okamoto, Koichiro Tsuji, Masaru Ohara, Yuichi Mine, Takahiro Shuto, Yuko Takamoto, Hiroki Nikawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
antimicrobial peptide
Cellular differentiation Core Binding Factor Alpha 1 Subunit Peptide lcsh:Chemistry Mice lcsh:QH301-705.5 MC3T3-E1 cells Spectroscopy Titanium chemistry.chemical_classification biology Chemistry Cell Differentiation General Medicine Computer Science Applications RUNX2 Biochemistry Porphyromonas gingivalis Histatins Article Catalysis Cell Line Inorganic Chemistry stomatognathic system Animals RNA Messenger Physical and Theoretical Chemistry titanium surface Molecular Biology Cell Proliferation Osteoblasts Cell growth Organic Chemistry Biofilm Alkaline Phosphatase equipment and supplies biology.organism_classification Molecular biology Immobilized Proteins lcsh:Biology (General) lcsh:QD1-999 Cell culture Biofilms Histatin Osteopontin Antimicrobial Cationic Peptides |
| Zdroj: | International Journal of Molecular Sciences, Vol 11, Iss 4, Pp 1458-1470 (2010) International Journal of Molecular Sciences Volume 11 Issue 4 Pages: 1458-1470 |
| Popis: | The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration. |
| Databáze: | OpenAIRE |
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