The alternative life of RNA-sequencing meets single molecule approaches
| Autor: | Sandra M. Fernández-Moya, Michael A. Kiebler, Janina Ehses |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Untranslated region Gene isoform Polyadenylation RNA Splicing Biophysics Nanotechnology Computational biology Biology Models Biological Biochemistry 03 medical and health sciences 0302 clinical medicine Structural Biology Genetics Humans RNA Messenger 3' Untranslated Regions Molecular Biology Gene Ribonucleoprotein Sequence Analysis RNA MRNA modification RNA Cell Biology 030104 developmental biology RNA splicing 030217 neurology & neurosurgery |
| Zdroj: | FEBS Letters. 591:1455-1470 |
| ISSN: | 0014-5793 |
| DOI: | 10.1002/1873-3468.12639 |
| Popis: | The central dogma of RNA processing has started to totter. Single genes produce a variety of mRNA isoforms by mRNA modification, alternative polyadenylation (APA), and splicing. Different isoforms, even those that code for the identical protein, may differ in function or spatiotemporal expression. One option of how this can be achieved is by the selective recruitment of trans-acting factors to the 3'-untranslated region of a given isoform. Recent innovations in high-throughput RNA-sequencing methods allow deep insight into global RNA regulation, whereas novel imaging-based technologies enable researchers to explore single RNA molecules during different stages of development, in different tissues and different compartments of the cell. Resolving the dynamic function of ribonucleoprotein particles in splicing, APA, or RNA modification will enable us to understand their contribution to pathological conditions. |
| Databáze: | OpenAIRE |
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