Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA
| Autor: | Heli Nummelin, Adrian Goldman, Mikael Skurnik, Yasmin El Tahir, Pauli Ollikka |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Protein Conformation
Gene Expression Yersinia medicine.disease_cause Crystallography X-Ray Bacterial genetics law.invention 03 medical and health sciences Protein structure Bacterial Proteins Structural Biology law Gene expression medicine Crystallization Adhesins Bacterial Escherichia coli 030304 developmental biology 0303 health sciences biology 030306 microbiology Chemistry General Medicine biology.organism_classification Molecular biology 3. Good health Bacterial adhesin Recombinant DNA bacteria Collagen |
| Zdroj: | Acta crystallographica. Section D, Biological crystallography. 58(Pt 6 Pt 2) |
| ISSN: | 0907-4449 |
| Popis: | A recombinant form of a collagen-binding fragment of Yersinia enterocolitica serotype O:3 adhesin YadA with an N-terminal polyhistidine affinity tag has been produced in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion technique. Crystals belong to the trigonal space group R3, with unit-cell parameters a = b = 67.05, c = 221.95 A, and diffract to 1.55 A resolution on a synchrotron-radiation source. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text