Contrasting splenic mechanisms in the blood clearance of red blood cells and colloidal particles

Autor:	M A, Klausner, L J, Hirsch, P F, Leblond, J K, Chamberlain, M R, Klemperer, G B, Segel
Rok vydání:	1975
Předmět:	Male Erythrocytes Cell Membrane Immunology Technetium Cell Biology Hematology History 18th Century Biochemistry Antibodies Carbon Chromium Radioisotopes Cortisone Mice Liver Phagocytosis Microscopy Electron Scanning Animals Colloids Spleen Sulfur
Zdroj:	Blood. 46:965-976
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v46.6.965.bloodjournal466965
Popis:	We have studied the blood clearance and organ uptake of colloidal particles and of antibody-coated and chemically treated Na2 51Cr O4- labeled erythrocytes (RBC) in mice. Hepatic and splenic uptake of both colloidal particles and autologous RBC coated with rabbit antibody were reduced significantly following pretreatment of animals with cortisone acetate. Hepatic removal of RBC previously treated in vitro with N- ethyl-maleimide (NEM) or phenylhydrazine-HCl (PHZ) was similarly depressed by pretreatment with cortisone. In contrast, the splenic uptake of NEM- and PHZ-altered erythrocytes was unaffected by cortisone. Scanning and transmission electron microscopic examination of perfused spleens from PHZ-injected animals demonstrated extensive mechanical trapping of Heinz body-containing RBC in sinus wall apertures, whereas little erythrophagocytosis was observed. These studies suggested that, while clearance of inert particulate matter and of antibody-coated RBC from the blood occurred primarily by a cortisone- suppressible, presumably phagocytic process in the spleen, chemically altered RBC were removed primarily by a cortisone-insensitive filtration process in the splenic microvasculature.
Databáze:	OpenAIRE
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