Usage of dried blood spots for molecular diagnosis and monitoring HIV-1 infection
| Autor: | Karoon Kuntiranont, Vipa Thiamchai, Suda Louisirirotchanakul, Ruengpung Sutthent, Sumonmal Uttayamakul, Sirirat Likanonsakul, Rujanee Sunthornkachit, Achara Chaovavanich, Sombat Tanprasertsuk |
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| Rok vydání: | 2005 |
| Předmět: |
Hiv seropositive
Human immunodeficiency virus (HIV) HIV Infections Biology medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Proviruses Pregnancy Virology Multiplex polymerase chain reaction medicine Humans Pregnancy Complications Infectious Dried blood Self-Sustained Sequence Replication Spots Infant Newborn Infant Thailand NASBA Infectious Disease Transmission Vertical Dried blood spot Blood DNA Viral HIV-1 Nucleic acid RNA Viral Female Reagent Kits Diagnostic |
| Zdroj: | Journal of Virological Methods. 128:128-134 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2005.04.010 |
| Popis: | The usage of dried blood spots as specimens for diagnosis and monitoring of HIV-1 infection in Thailand was evaluated. EDTA blood samples, which were collected from 100 HIV seronegative and 109 HIV seropositive individuals, were tested on dried blood spots; Whatman, Schleicher and Schuell (S&S) No. 903 and S&S IsoCode filter paper. Nucleic acid was extracted and used as a template for HIV-1 proviral DNA detection by an “in-house” multiplex PCR and a commercial Amplicor HIV-1 PCR test (Roche, version 1.0). HIV-1 RNA qualitative (QL) and quantitative (QT) detection was determined by Nucleic Acid Sequence Based Amplification (NASBA). The average DNA per blood spot recovered from Whatman and S&S IsoCode was not statistically different (p = 0.512) with a range of 218.9 ± 46.84 and 225.63 ± 88.33 μg, respectively. The concordance of HIV-1 proviral DNA detection by PCR from dried blood spots Whatman and S&S IsoCode was 94% versus 89.4% for sensitivity and 100% versus 100% for specificity. The sensitivity and specificity of HIV-1 RNA QL detection in dried blood spots was 89.7 and 97.5%, respectively. The HIV-1 RNA QT from dried blood spots showed a good correlation in paired dried blood spots and plasma with Pearson correlation, r = 0.817 (R2 = 0.667, P |
| Databáze: | OpenAIRE |
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