Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch
Autor: | Rui Xue Zhang, Junjun She, Yao Ma, Zhenhong Pan, Gaixia Liu, Mingxia Zhang, Qingxia Wei, Hongping Xia, Junhong Liu, Yaguang Li |
---|---|
Rok vydání: | 2019 |
Předmět: |
Male
medicine.medical_specialty Colorectal cancer Colon General Chemical Engineering Azoxymethane Gut flora Adenocarcinoma digestive system Inflammatory bowel disease Gastroenterology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Colon surgery Internal medicine Medicine Animals Appendectomy Colitis Cecum General Immunology and Microbiology biology business.industry General Neuroscience Dextran Sulfate medicine.disease biology.organism_classification digestive system diseases Appendix Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure chemistry Chronic Disease Carcinogens business Colorectal Neoplasms |
Zdroj: | Journal of visualized experiments : JoVE. (150) |
ISSN: | 1940-087X |
Popis: | The human appendix has been recently implicated to play important biological roles in the pathogenesis of various complex diseases, such as colorectal cancer, inflammatory bowel disease, and Parkinson’s disease. To study the function of the appendix, a gut disease-associated murine appendectomy model has been established and its step-by-step protocol is described here. This report introduces a facile protocol for caecal patch removal in mice followed by the chemical induction of chronic colitis-associated colorectal cancer using a combination of dextran sulfate sodium (DSS) and azoxymethane (AOM). IgA specific cells and IgA concentration were significantly reduced upon removal of the caecal patch in male C57BL/6 mice compared to those in the sham group. Simultaneously administering 2% DSS and AOM resulted in nearly 80% mice survival in both sham and appendectomy groups without significant body weight loss. Histological results confirmed colonic inflammation and different degrees of adenocarcinoma. This model can be used for the study of the functional role of the appendix in maintaining gut microbiota homeostasis and pathogenesis of gut colitis and malignancies, as well as for the potential development of drug targeting therapies. |
Databáze: | OpenAIRE |
Externí odkaz: |