Mating induces early transcriptional response in the rat endosalpinx: the role of TNF and RA
Autor: | Rodrigo A. Gutiérrez, Juan-Carlos Andrade, Francisca Fábrega-Guerén, Horacio B. Croxatto, Ethel V. Velasquez, Lidia M. Zuñiga, Patricio Morales, Pedro A. Orihuela, Elena A. Vidal, Benito Gómez-Silva |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Male Embryology Microarray Receptors Retinoic Acid Retinoic acid Tretinoin Oviducts Biology Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Sexual Behavior Animal 0302 clinical medicine Endocrinology Downregulation and upregulation medicine Animals Mating Gene reproductive and urinary physiology 030219 obstetrics & reproductive medicine Mucous Membrane Tumor Necrosis Factor-alpha Embryogenesis Obstetrics and Gynecology Cell Biology Oocyte Cell biology 030104 developmental biology medicine.anatomical_structure Reproductive Medicine chemistry Gene Expression Regulation Oviduct Female Transcriptome |
Zdroj: | Reproduction (Cambridge, England). 161(1) |
ISSN: | 1741-7899 |
Popis: | During mating, males provide not only the spermatozoa to fertilize the oocyte but also other stimuli that are essential for initiating and maintaining the reproductive programme in females. In the mammalian oviduct, mating regulates sperm storage, egg transport, fertilization, early embryonic development, and oestradiol metabolism. However, the main molecules underlying these processes are poorly understood. Using microarray analyses, we identified 58 genes that were either induced or repressed by mating in the endosalpinx at 3 h post-stimulus. RT-qPCR confirmed that mating downregulated the expression of the Oas1h and Prim1 genes and upregulated the expression of the Ceacam1, Chad, Chst10, Slc5a3 and Slc26a4 genes. The functional category ‘cell-to-cell signalling and interaction’ was over-represented in this gene list. Network modelling identified TNF and all-trans retinoic acid (RA) as upstream regulators of the mating-induced transcriptional response, which was confirmed by intraoviductal injection of TNF or RA in unmated rats. It partially mimicked the transcriptional effect of mating in the rat endosalpinx. Furthermore, mating decreased RA levels in oviductal fluid, and RA-receptor-gamma (RARG) exhibited a nuclear location in oviductal epithelium in both unmated and mated rats, indicating RA-RARG transcriptional activity. In conclusion, the early transcriptional response regulated by mating in the rat endosalpinx is mediated by TNF and RA. These signalling molecules regulate a cohort of genes involved in ‘cell-to-cell signalling and interactions’ and merit further studies to understand the specific processes activated in the endosalpinx to sustain the events that occur in the mammalian oviduct early after mating. |
Databáze: | OpenAIRE |
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