Isolation and structural analyses of positional isomers of 61,6m-di-O-α-d-mannopyranosyl-cyclomaltooctaose (m=2–5) and 6-O-α-(n-O-α-d-mannopyranosyl)-α-d-mannopyranosyl-cyclomaltooctaose (n=2, 3, 4, and 6)
| Autor: | Kazuha Matsuda, Kyoko Koizumi, Hitoshi Hashimoto, Yasuyo Okada, Sumio Kitahata, Kenichi Hamayasu |
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| Rok vydání: | 1998 |
| Předmět: |
Cyclodextrins
Magnetic Resonance Spectroscopy Plants Medicinal Chemistry Stereochemistry Molecular Sequence Data Organic Chemistry Fabaceae Stereoisomerism General Medicine Nuclear magnetic resonance spectroscopy Spectrometry Mass Fast Atom Bombardment Fast atom bombardment Condensation reaction Biochemistry High-performance liquid chromatography Analytical Chemistry Carbohydrate Sequence Carbohydrate Conformation Structural isomer Carbohydrate conformation Chromatography High Pressure Liquid Cyclomaltodextrin glucanotransferase |
| Zdroj: | Carbohydrate Research. 310:229-238 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/s0008-6215(98)00187-6 |
| Popis: | Eight positional isomers of 6(1),6m-di-O-alpha-D-mannopyranosyl-cyclomaltooctaose (gamma CD) (m = 2-5) and 6-O-alpha-(n-O-alpha-D-mannopyranosyl)-D-mannopyranosyl-gamma CD (n = 2, 3, 4, and 6) in a mixture of products from gamma CD and D-mannose by condensation reaction of alpha-mannosidase from jack bean were isolated by HPLC. The structures of four isomers of 6-O-alpha-(n-O-alpha-D-mannopyranosyl)-D-mannopyranosyl-gamma CD were elucidated by NMR spectroscopy. On the other hand, four positional isomers of 6(1),6m-di-O-alpha-D-mannopyranosyl-gamma CD were determined by LC-MS analysis of degree of polymerization of the branched oligosaccharides produced by enzymatic degradation with bacterial saccharifying alpha-amylase (BSA), and combination of BSA and glucoamylase. Similarly cyclomaltodextrin glucanotransferase also digested these isomers. |
| Databáze: | OpenAIRE |
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