Viral hepatitis in the Peruvian Amazon: Ethnomedical context and phytomedical resource

Autor: Hivelli Ericka Ricopa Cotrina, Vincent Roumy, Thierry Hennebelle, Jean Dubuisson, Lastenia Ruiz, Sébastien Anthérieu, Andréa-Luz Gutierrez-Choquevilca, Céline Rivière, Leonor Arévalo Encinas, Wilfredo Ruiz Mesía, Karin Séron, Sevser Sahpaz, Guillaume Garçon, Jennifer Samaillie, Juan Celidonio Ruiz Macedo, S Bordage
Přispěvatelé: Institut Charles Viollette (ICV) - ULR 7394 (ICV), Université d'Artois (UA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratorio de Investigación de Productos Naturales Antiparasitarios de la Amazonia (LIPNAA), Centro de Investigaciones de Recursos Naturales de la Amazonía (CIRNA), Universidad Nacional de la Amazonía Peruana [Loreto, Perou] (UNAP)-Universidad Nacional de la Amazonía Peruana [Loreto, Perou] (UNAP), Universidad Nacional de la Amazonía Peruana [Loreto, Perou] (UNAP), Herbarium de la Amazonía Peruana Amazonense de la Universidad Nacional de la Amazonía Peruana ( UNAP), Laboratoire d'anthropologie sociale (LAS), École des hautes études en sciences sociales (EHESS)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Impact de l'environnement chimique sur la santé humaine - ULR 4483 (IMPECS), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Members and contributors of the scientific humanitarian association ARPIA (Association for Research on Indigenous Amazonian Pharmacopeia), F.M. Lambert (Commission du Développement durable et de l'Aménagement du territoire), Gaël Ostyn, CPER Alibiotech, C. Mori and C. Güivinsinti, Luis and Aurelio Chino (presidents of Indigenous Quechua Federations), the members of the Quechua community 'Sabalo Yacu' who contributed to the building of a traditional medical centre in the Alto Pastaza., Séron, Karin
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Rainforest
Medicinal Plant
medicine.medical_treatment
[SDV]Life Sciences [q-bio]
Context (language use)
Hepacivirus
Biology
Antiviral Agents
Virus
Targeted therapy
[SHS]Humanities and Social Sciences
03 medical and health sciences
0302 clinical medicine
Amazonia
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Antiviral Activity
Drug Discovery
Peru
medicine
Humans
Cytotoxicity
030304 developmental biology
Traditional Use
Pharmacology
Hepatitis
0303 health sciences
Plants
Medicinal
Traditional medicine
Plant Extracts
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Hepatitis C
Hep G2 Cells
[SHS.ANTHRO-SE]Humanities and Social Sciences/Social Anthropology and ethnology
medicine.disease
[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
3. Good health
030220 oncology & carcinogenesis
[SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Toxicity
Ethnopharmacology
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Viral hepatitis
Phytotherapy
Zdroj: Journal of Ethnopharmacology
Journal of Ethnopharmacology, 2020, 255, pp. 112735. ⟨10.1016/j.jep.2020.112735⟩
Journal of Ethnopharmacology, Elsevier, 2020, 255, pp.112735. ⟨10.1016/j.jep.2020.112735⟩
Journal of Ethnopharmacology, 2020, 255, pp.112735. ⟨10.1016/j.jep.2020.112735⟩
ISSN: 0378-8741
DOI: 10.1016/j.jep.2020.112735⟩
Popis: International audience; Ethnopharmacological relevance: An extensive ethnopharmacological survey was carried out in the Peruvian Amazonian district of Loreto with informants of various cultural origins from the surroundings of Iquitos (capital city of Loreto) and from 15 isolated riverine Quechua communities of the Pastaza River.A close attention was paid to the medical context and plant therapy, leading to the selection of 35 plant species (45 extracts). The extracts were tested for antiviral activity against HCV with counting of Huh-7 cellular death in case of toxicity, and cytotoxicity was evaluated in HepG2 cells.Aim of the study: The aim of the study was to inventory the plants used against hepatitis in Loreto, then to evaluate their antiviral activity and to suggest a way to improve local therapeutic strategy against viral hepatitis, which is a fatal disease that is still increasing in this area.Materials and methods: An ethnographic survey was carried out using “participant-observation” methodology and focusing on plant therapy against hepatitis including associated remedies. 45 parts of plant were extracted with methanol and tested in vitro for anti-HCV activity in 96-well plate, using HCV cell culture system with immunofluorescent detection assisted by automated confocal microscopy. Toxicity of plant extracts was also evaluated in microplates on hepatic cells by immunofluorescent detection, for the Huh-7 nuclei viability, and by UV-absorbance measurement of MTT formazan for cytotoxicity in HepG2 cells.Results: In vitro assay revealed interesting activity of 18 extracts (50% infection inhibition at 25 μg/mL) with low cytotoxicity for 15 of them. Result analysis showed that at least 30% of HCV virus were inhibited at 25 μg/mL for 60% of the plant extracts. Moreover, the ethnomedical survey showed that remedies used with low and accurate dosing as targeted therapy against hepatitis are usually more active than species indicated with more flexible dosing to alleviate symptoms of hepatic diseases.Conclusion: Together with bibliographic data analysis, this study supported the traditional medicinal uses of many plants and contributed to a better understanding of the local medical system. It also permitted to refine the therapeutic plant indications regarding patients’ liver injuries and vulnerability. Only 2 of the 15 most active plant species have already been studied for antiviral activity against hepatitis suggesting new avenues to be followed for the 13 other species.
Databáze: OpenAIRE
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