Differential susceptibility to acute Trypanosoma cruzi infection in BALB/c and C57BL/6 mice is not associated with a distinct parasite load but cytokine abnormalities
| Autor: | Eduardo Roggero, Oscar Bottasso, M. Tamae-Kakazu, Ana María Cánovas Pérez, Esteban Serra, Silvia Revelli, Juana Wietzerbin, Isabel Piazzon, Irene Nepomnaschy |
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| Rok vydání: | 2002 |
| Předmět: |
C57BL/6
CD4-Positive T-Lymphocytes Chagas Cardiomyopathy Lipopolysaccharides Male Time Factors medicine.medical_treatment Apoptosis CD8-Positive T-Lymphocytes Parasitemia Parasite load purl.org/becyt/ford/1 [https] Mice Immunology and Allergy Cells Cultured Mice Inbred BALB C biology Cytokine Acute Disease Cytokines Female Disease Susceptibility CIENCIAS NATURALES Y EXACTAS Chagas disease Otras Ciencias Biológicas Trypanosoma cruzi Immunology Thymus Gland Nitric Oxide BALB/c Ciencias Biológicas Animal model Species Specificity parasitic diseases Weight Loss medicine Animals Chagas Disease Genetic Predisposition to Disease purl.org/becyt/ford/1.6 [https] Original Articles biology.organism_classification medicine.disease Virology APOPTOSIS SUSCEPTIBILITY CYTOKINE IMBALANCES T. CRUZI THYMUS ATROPHY Mice Inbred C57BL Disease Models Animal Trypanosomiasis |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 0009-9104 |
| Popis: | Inoculation of Trypanosoma cruzi, Tulahuén strain, into C57BL/6 and BALB/c mice led to an acute infection characterized by marked parasitaemia, myocardial inflammation and thymocyte depletion. While C57BL/6 mice showed a progressive and lethal disease, BALB/c mice partly recovered. To characterize these murine models more effectively, we studied the parasite burden, serum levels of major infection outcome-related cytokines, thein vitro features of T. cruzi infection in peritoneal macrophages and the immunophenotype of thymic cells. The greater disease severity of T. cruzi-infected C57BL/6 mice was not linked to an increased parasite load, as parasitaemia, myocardial parasite nests and amastigote counts in peritoneal macrophages were not different from those in BALB/c mice. Cortical thymocyte loss was accompanied by the presence of apoptotic bodies and fragmented nuclear DNA, whereas fluorocytometric analysis at 17 days postinfection (p.i.) revealed a more pronounced loss of CD4 + CD8 + cells in C57BL/6 mice. This group displayed higher levels of TNF-α on days 14 and 21 p.i., in the presence of lower IL-1β and IL-10 concentrations by days 14 and 21, and days 7 and 14 p.i., respectively. Day-21 evaluation showed higher concentrations of nitrate and TNF-α soluble receptors in C57BL/6 mice with no differences in IFN-γ levels, with respect to the BALB/c group. Increased morbidity of C57BL/6 T. cruzi-infected mice does not seem to result from an aggravated infection but from an unbalanced relationship between pro- and anti-inflammatory mediators. Fil: Roggero, Eduardo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina Fil: Perez, Ana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina Fil: Tamae Kakazu, M.. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina Fil: Piazzon, Margarita Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina Fil: Nepomnaschy, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina Fil: Wietzerbin, J.. Inserm; Francia Fil: Serra, Esteban Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Revelli, Silvia Susana. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina Fil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina |
| Databáze: | OpenAIRE |
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