Single-Cell RNA Sequencing of Arabidopsis Leaf Tissues Identifies Multiple Specialized Cell Types: Idioblast Myrosin Cells and Potential Glucosinolate-Producing Cells
| Autor: | Taro Maeda, Shigeo S Sugano, Makoto Shirakawa, Mayu Sagara, Toshiro Ito, Satoshi Kondo, Atsushi J Nagano |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Plant and Cell Physiology. 64:234-247 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcac167 |
| Popis: | The glucosinolate–myrosinase defense system (GMDS), characteristic of Brassicales, is involved in plant defense. Previous single-cell transcriptomic analyses have reported the expression profiles of multiple GMDS-related cell types (i.e. myrosinase-rich myrosin idioblasts and multiple types of potential glucosinolate synthetic cells as well as a candidate S-cell for glucosinolate accumulation). However, differences in plant stages and cell-type annotation methods have hindered comparisons among studies. Here, we used the single-cell transcriptome profiles of extended Arabidopsis leaves and verified the distribution of previously used markers to refine the expression profiles of GMDS-associated cell types. Moreover, we performed beta-glucuronidase promoter assays to confirm the histological expression patterns of newly obtained markers for GMDS-associated candidates. As a result, we found a set of new specific reporters for myrosin cells and potential glucosinolate-producing cells. |
| Databáze: | OpenAIRE |
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