A Universal DNA-Based Protein Detection System

Autor: Songming Peng, Mark R. Hartman, Faping Duan, Jinhui Cui, Dayong Yang, John T. Lis, John C. March, Thua N. N. Tran, Haixin Cui, Hisakage Funabashi, Dan Luo
Rok vydání: 2013
Předmět:
Zdroj: Journal of the American Chemical Society. 135:14008-14011
ISSN: 1520-5126
0002-7863
DOI: 10.1021/ja405872g
Popis: Protein immune detection requires secondary antibodies which must be carefully selected in order to avoid interspecies cross-reactivity, and is therefore restricted by the limited availability of primary/secondary antibody pairs. Here we present a versatile DNA-based protein detection system using a universal adapter to interface between IgG antibodies and DNA-modified reporter molecules. As a demonstration of this capability, we successfully used DNA nano-barcodes, quantum dots, and horseradish peroxidase enzyme to detect multiple proteins using our DNA-based labeling system. Our system not only eliminates secondary antibodies but also serves as a novel method platform for protein detection with modularity, high capacity, and multiplexed capability.
Databáze: OpenAIRE