Harnessing CRISPR Effectors for Infectious Disease Diagnostics
| Autor: | Sri Gowtham Thakku, Roby P. Bhattacharyya, Deborah T. Hung |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Bacteria Effector Loop-mediated isothermal amplification 02 engineering and technology Computational biology Biology 021001 nanoscience & nanotechnology Communicable Diseases Bacterial genetics 03 medical and health sciences 030104 developmental biology Infectious Diseases Viral genetics Infectious disease (medical specialty) DNA Viral Viruses Humans CRISPR Clustered Regularly Interspaced Short Palindromic Repeats 0210 nano-technology Pathogen Nucleic acid detection |
| Zdroj: | ACS Infectious Diseases. 4:1278-1282 |
| ISSN: | 2373-8227 |
| DOI: | 10.1021/acsinfecdis.8b00170 |
| Popis: | Nucleic acid detection is an important method for pathogen identification but can be expensive, have variable sensitivity and specificity, and require substantial infrastructure. Two new methods capitalize on unexpected in vitro properties of clustered regularly interspaced short palindromic repeats (CRISPR) effectors, turning activated nucleases into intrinsic amplifiers of a specific nucleic-acid binding event. These effectors are coupled with a variety of reporters and used in tandem with existing isothermal amplification methods to produce sensitive, sequence-specific pathogen identification in multiple field-deployable formats. While still in their infancy, these modular CRISPR-based methods have the potential to transform pathogen identification and other aspects of infectious disease diagnostics. |
| Databáze: | OpenAIRE |
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