Cellular origin and diagnostic significance of high-fluorescent cells in cerebrospinal fluid detected by the XE-5000 hematology analyzer
| Autor: | J.-B. Gonzalez, Klemens Ruprecht, Mathias Zimmermann, Stefan Prokop, Carolin Otto |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Cytodiagnosis Clinical Biochemistry Cell Count Tumor cells Intrathecal Likelihood ratios in diagnostic testing Young Adult Cerebrospinal fluid Cellular origin Hematology analyzer Humans Medicine Aged Cerebrospinal Fluid Aged 80 and over Body fluid Microscopy business.industry Biochemistry (medical) Hematology General Medicine Middle Aged Flow Cytometry Fluorescence ROC Curve Female business |
| Zdroj: | International Journal of Laboratory Hematology. 35:580-588 |
| ISSN: | 1751-5521 |
| Popis: | Summary Introduction The Sysmex XE-5000 is a blood and body fluid analyzer able to differentiate cells into polymorphonuclear, mononuclear, and high-fluorescent cells (HFC). The identity of HFC in cerebrospinal fluid (CSF) has been uncertain; however, compatible with their high nucleic acid content, HFC could represent intrathecal tumor cells. Here, we studied the cellular origin and the diagnostic significance of HFC in CSF. Methods Results of CSF examinations with the XE-5000 were analyzed in 65 CSF samples with and 126 CSF samples without tumor cells, as defined by manual microscopy of CSF cytospin preparations. Results The XE-5000 detected HFC in 51 of 65 tumor cell–positive and in 33 of 126 tumor cell–negative CSF samples (sensitivity: 78.5%, specificity: 73.8%, positive likelihood ratio: 3.0, negative likelihood ratio: 0.29). The percentages of HFC and tumor cells in CSF samples correlated (r² = 0.41, P |
| Databáze: | OpenAIRE |
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