cDNA cloning and localization of Sp3111 (also called Ms4a14) in the rat testis
| Autor: | Yihua Gu, Michelle Santos, Ching-Ling C Chen, Yong-Mei Chen, Miao Liu, Yan Xu, Huijuan Shi, Xiao-feng Jia, Xiao-dong Zhang, Ai-Zhen Wu |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Embryology Zygote In situ hybridization Biology Rats Sprague-Dawley Mammalian reproduction Endocrinology Complementary DNA Testis Animals RNA Messenger Cloning Molecular Acrosome In Situ Hybridization Cloning Messenger RNA Gene Expression Regulation Developmental Membrane Proteins Obstetrics and Gynecology RNA Sequence Analysis DNA Cell Biology Immunohistochemistry Spermatids Spermatozoa Cell biology Meiosis Reproductive Medicine Fertilization Sperm Midpiece |
| Zdroj: | REPRODUCTION. 148:81-86 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-14-0087 |
| Popis: | With tetraspanning topology, members of the membrane-spanning four-domain subfamily A (MS4A) may facilitate signaling or ion channel functions in many tissues. In this study, we report the cloning of a full-length cDNA from rat testis, designatedMs4a14(Sp3111), which encodes the MS4A protein with 1139 amino acid residues.In situhybridization and immunohistochemical analyses indicate thatMs4a14is predominantly expressed from round spermatids to spermatozoa at specific stages in the rat testis at both the mRNA and protein level. Immunofluorescence analysis revealed that MS4A14 (SP3111) is located in the acrosome and the midpiece of the flagellum in mature sperm. Previously, we explored and reported the involvement of MS4A14 in reproductive functions, using antibody blockage during IVF and a transgenic RNA interference method in a mouse model. Our results suggested that MS4A14 is involved in fertilization and zygote division. As MS4A14 protein exists in mammals, such as humans, cows, dogs, and rodents, MS4A14 may play a ubiquitous role in mammalian reproduction. |
| Databáze: | OpenAIRE |
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