Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine
| Autor: | Nicolas Kouttab, Abby Maizel, Douglas M. Gersten, David Shrayer, Vincent J. Hearing, Harold J. Wanebo |
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| Rok vydání: | 1993 |
| Předmět: |
Cytotoxicity
Immunologic Cancer Research Antibodies Neoplasm Fibrosarcoma Melanoma Experimental Active immunization Melanoma Vaccine Mice Antigen Antigens Neoplasm Formaldehyde Tumor Cells Cultured Animals Cytotoxic T cell Cytotoxicity Vaccines biology Virology In vitro Neoplasm Proteins Mice Inbred C57BL Molecular Weight Oncology Antigens Surface Humoral immunity Immunology biology.protein Antibody |
| Zdroj: | International Journal of Cancer. 53:696-702 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.2910530428 |
| Popis: | The goal of our experiments was to determine the extent to which the humoral response to a melanoma vaccine elicits the production of cytotoxic antibodies in tumor-challenged mice. Mice were immunized with a vaccine produced from formalinized extracellular antigens (FECA) derived from B16 F10 melanomas. The production of antibodies that recognized the vaccine preparation was determined by ELISA, as was their cross-reactivity with the B700 melanoma antigen. The antibodies were shown to be anti-proliferative by inhibition of tritiated thymidine incorporation into the DNA of cultured target cells and cytotoxic by assays for complement-mediated and antibody-dependent cellular cytotoxicity. Flow cytometric analyses indicated that approximately 60% of the target cells specifically bound antibody from the immune sera. These results confirm that B700 is a significant antigenic component of the FECA vaccine, and provide encouragement for this approach to developing useful melanoma vaccines. |
| Databáze: | OpenAIRE |
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