The impact of hyperglycemia and the presence of encapsulated islets on oxygenation within a bioartificial pancreas in the presence of mesenchymal stem cells in a diabetic Wistar rat model
| Autor: | Marie-Sophie Paridaens, Sophie Vériter, Denis Dufrane, Najima Aouassar, Bernard Gallez, Pierre Gianello, Bénédicte F. Jordan, Pierre-Yves Adnet, Oussama Karroum, Charlotte Stuckman |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Materials science Swine Biophysics Islets of Langerhans Transplantation Adipose tissue Bioengineering Diabetes Mellitus Experimental Biomaterials chemistry.chemical_compound In vivo Internal medicine medicine Animals Rats Wistar Pancreas geography geography.geographical_feature_category Bioartificial Organs Mesenchymal stem cell Mesenchymal Stem Cells Islet Flow Cytometry Rats Transplantation Vascular endothelial growth factor Endocrinology medicine.anatomical_structure chemistry Mechanics of Materials Hyperglycemia Ceramics and Composites Bone marrow Stem cell |
| Zdroj: | Biomaterials. 32(26) |
| ISSN: | 1878-5905 |
| Popis: | This study investigates the potential of bone marrow (BM-MSCs) versus adipose mesenchymal stem cells (AMSCs) to potentiate the oxygenation of encapsulated islets in a subcutaneous bioartificial pancreas. Oxygen pressures (inside subcutaneous implants) were followed in vivo (by electronic paramagnetic resonance) in non-diabetic/diabetic rats transplanted with encapsulated porcine islets or empty implants up to 4 weeks post-transplantation. After graft explantation, neoangiogenesis surrounding the implants was assessed by histomorphometry. Angiogenic properties of BM-MSCs and AMSCs were first assessed in vitro by incubation of the cells in hypoxia chambers, under normoxic/hypoxic and hypo-/hyperglycemic conditions, followed by quantification of vascular endothelial growth factor (VEGF) release. Second, the in vivo aspect was studied by subcutaneous transplantation of encapsulated BM-MSCs and AMSCs in diabetic rats and assessment of the cells' angiogenic properties as described above. Diabetic state and islet encapsulation induced a significant decrease of oxygenation of the subcutaneous implant and an increased number of cells expressing VEGF. AMSCs demonstrated a significantly higher VEGF secretion than BM-MSCs in vitro. In vivo, AMSCs improved the implant's oxygenation and vascularization. Diabetes and islet encapsulation significantly reduced the oxygenation of a subcutaneous bioartificial pancreas. AMSCs can improve oxygenation by VEGF release in hypoxia and hyperglycemia states. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect