Fabrication of an immunosensor for early and ultrasensitive determination of human tissue plasminogen activator (tPA) in myocardial infraction and breast cancer patients

Autor:	Khosro Piri, Haidar Saify Nabiabad, Abbas Afkhami, Fatemeh Kafrashi, Tayyebeh Madrakian
Rok vydání:	2018
Předmět:	Models Molecular Working electrode medicine.drug_class Myocardial Infarction Breast Neoplasms Biosensing Techniques 02 engineering and technology Glassy carbon 010402 general chemistry Monoclonal antibody 01 natural sciences Biochemistry Tissue plasminogen activator Analytical Chemistry Limit of Detection Biomarkers Tumor medicine Humans Early Detection of Cancer Immunoassay Detection limit Gel electrophoresis Chromatography Nanotubes Carbon Chemistry Reproducibility of Results Electrochemical Techniques Middle Aged 021001 nanoscience & nanotechnology 0104 chemical sciences Tissue Plasminogen Activator Female Cyclic voltammetry 0210 nano-technology Antibodies Immobilized Biosensor medicine.drug
Zdroj:	Analytical and Bioanalytical Chemistry. 410:3683-3691
ISSN:	1618-2650 1618-2642
Popis:	Sensitive detection of biomarkers will mean accurate and early diagnosis of diseases. A tissue plasminogen activator (tPA) has a crucial role in many cardiovascular diseases and it is related to many processes such as angiogenesis in cancer cells. Therefore, sensitive determination of tPA is important in diagnosis and clinical research. tPA monoclonal antibody was covalently attached onto single-wall carbon nanotubes (SWCNTs) using diimide-activated imidation coupling. Functionalized SWCNTs were immobilized onto a glassy carbon electrode and the modification process was investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), SEM, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Cyclic voltammograms (CVs) in a scan rate of 100 mVs−1 was studied and comparisons were made between the modified glassy carbon electrodes (immobilized with antibodies) as a working electrode before and after the formation of tPA-antibody complex. Results of the SDS-PAGE demonstrated that the antibody was covalently and site directly attached to the SWCNTs. The fabricated biosensor provided a good linear response range from 0.1 to 1.0 ng mL−1 with a low detection limit of 0.026 ng mL−1. The immunosensor showed selectivity, reproducibility, good sensitivity, and acceptable stability. Satisfactory results were observed for early and sensitive determination of tPA in human serum samples. For the first time, such specific biosensor is currently being fabricated for tPA in our laboratories and successfully could determine tPA in myocardial infraction and breast cancer patients.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::362fe135f856189bc5c4af8bff52544b https://doi.org/10.1007/s00216-018-1005-y Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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