E2F1 induces apoptosis and sensitizes human lung adenocarcinoma cells to death-receptor-mediated apoptosis through specific downregulation of c-FLIP(short)
| Autor: | Elisabeth Brambilla, Laurence Chaperot, Christian Brambilla, Olivier Micheau, Joel Plumas, Sylvie Gazzeri, Caroline Salon, Beatrice Eymin |
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| Přispěvatelé: | Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Mort cellulaire et cancer, Université de Bourgogne (UB)-IFR100 - Structure fédérative de recherche Santé-STIC-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Michallon, INSERM U823, équipe 2 (Bases Moléculaires de la Progression des Cancers du Poumon), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut d'oncologie/développement Albert Bonniot de Grenoble ( INSERM U823 ), Université Joseph Fourier - Grenoble 1 ( UJF ) -CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université de Bourgogne ( UB ) -IFR100 - Structure fédérative de recherche Santé-STIC-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Etablissement Français du Sang Rhône-Alpes, EFS, Université Joseph Fourier - Grenoble 1 ( UJF ) -CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Joseph Fourier - Grenoble 1 ( UJF ) -CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Salas, Danielle |
| Rok vydání: | 2005 |
| Předmět: |
MESH: CASP8 and FADD-Like Apoptosis Regulating Protein
MESH : RNA Messenger Lung Neoplasms MESH : DNA MESH : Cytotoxicity Immunologic MESH: Membrane Glycoproteins CASP8 and FADD-Like Apoptosis Regulating Protein Apoptosis MESH : Blotting Western MESH : Fluorescent Antibody Technique Indirect MESH: Down-Regulation MESH : E2F1 Transcription Factor [ SDV.CAN ] Life Sciences [q-bio]/Cancer TNF-Related Apoptosis-Inducing Ligand MESH : Adenocarcinoma 0302 clinical medicine Cytotoxic T cell Fluorescent Antibody Technique Indirect 0303 health sciences Membrane Glycoproteins MESH : Reverse Transcriptase Polymerase Chain Reaction Intracellular Signaling Peptides and Proteins MESH: Tumor Necrosis Factors Cell biology Gene Expression Regulation Neoplastic 030220 oncology & carcinogenesis Caspases Adenocarcinoma Tumor necrosis factor alpha MESH: TNF-Related Apoptosis-Inducing Ligand endocrine system MESH: Enzyme Activation MESH : Gene Expression Regulation Neoplastic Blotting Western Down-Regulation MESH : T-Lymphocytes Cytotoxic 03 medical and health sciences [SDV.CAN] Life Sciences [q-bio]/Cancer MESH: Blotting Western Humans MESH : Lung Neoplasms RNA Messenger Molecular Biology MESH: Humans MESH: Caspases Tumor Necrosis Factor-alpha MESH : Humans DNA medicine.disease MESH: Lung Neoplasms Enzyme Activation MESH: Tumor Necrosis Factor-alpha Apoptosis Regulatory Proteins MESH : Apoptosis T-Lymphocytes Cytotoxic Cytotoxicity Immunologic MESH : Membrane Glycoproteins MESH : Fas Ligand Protein MESH: Caspase 8 Fas ligand MESH : Down-Regulation MESH : TNF-Related Apoptosis-Inducing Ligand MESH: Reverse Transcriptase Polymerase Chain Reaction E2F1 MESH : Tumor Necrosis Factor-alpha Caspase 8 MESH : Caspase 8 Reverse Transcriptase Polymerase Chain Reaction MESH: DNA MESH: Gene Expression Regulation Neoplastic MESH: E2F1 Transcription Factor Tumor Necrosis Factors biological phenomena cell phenomena and immunity MESH : Apoptosis Regulatory Proteins Programmed cell death MESH: Cell Line Tumor Fas Ligand Protein MESH : Tumor Necrosis Factors [SDV.CAN]Life Sciences [q-bio]/Cancer Biology Downregulation and upregulation MESH : Intracellular Signaling Peptides and Proteins MESH: Intracellular Signaling Peptides and Proteins Cell Line Tumor medicine MESH: Fluorescent Antibody Technique Indirect MESH: Cytotoxicity Immunologic 030304 developmental biology MESH: RNA Messenger MESH : Cell Line Tumor MESH: Apoptosis Regulatory Proteins MESH: Apoptosis MESH: Adenocarcinoma Cell Biology MESH : CASP8 and FADD-Like Apoptosis Regulating Protein MESH: Fas Ligand Protein Cancer research MESH : Caspases MESH : Enzyme Activation MESH: T-Lymphocytes Cytotoxic E2F1 Transcription Factor |
| Zdroj: | Cell Death and Differentiation Cell Death and Differentiation, Nature Publishing Group, 2006, 13 (2), pp.260-72. ⟨10.1038/sj.cdd.4401739⟩ Cell Death and Differentiation, Nature Publishing Group, 2006, 13 (2), pp.260-72. 〈10.1038/sj.cdd.4401739〉 |
| ISSN: | 1350-9047 1476-5403 |
| DOI: | 10.1038/sj.cdd.4401739⟩ |
| Popis: | E2F1 is a transcription factor that plays a well-documented role during S phase progression and apoptosis. We had previously postulated that the low level of E2F1 in primary lung adenocarcinoma contributes to their carcinogenesis. Here, we show that E2F1 triggers apoptosis in various lung adenocarcinoma cell lines by a mechanism involving the specific downregulation of the cellular FLICE-inhibitory protein short, leading to caspase-8 activation at the death-inducing signaling complex. Importantly, we also provide evidence that E2F1 sensitizes tumor as well as primary cells to apoptosis mediated by FAS ligand or tumor necrosis factor-related apoptosis-inducing ligand, and enhances the cytotoxic effect of T lymphocytes against tumor cells. Finally, we describe the specific overexpression of c-FLIP(S) in human lung adenocarcinomas with low level of E2F1. Overall, our data identify E2F1 as a critical determinant of the cellular response to death-receptor-mediated apoptosis, and suggest that its downregulation contributes to the immune escape of lung adenocarcinoma tumor cells. |
| Databáze: | OpenAIRE |
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