Hepatoprotective Effects of Standardized Extracts from an Ancient Italian Apple Variety (Mela Rosa dei Monti Sibillini) against Carbon Tetrachloride (CCl4)-Induced Hepatotoxicity in Rats

Autor: Mohammad Amin Sadeghi, Hasan Yousefi-Manesh, Stefano Dall'Acqua, Samira Shirooie, Ahmad Reza Dehpour, Giovanni Caprioli, Sara Hemmati, Reza Hashemi Shahraki, Joice Guileine Nkuimi Wandjou, Stefania Sut, Sedighe Ansari-Nasab, Filippo Maggi, Seyed Mohammad Nabavi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
CCl4
Antioxidant
antioxidant
Biopsy
medicine.medical_treatment
Anti-Inflammatory Agents
Pharmaceutical Science
Analytical Chemistry
chemistry.chemical_compound
0302 clinical medicine
Oral administration
Drug Discovery
Carbon Tetrachloride
anti-inflammatory
0303 health sciences
Molecular Structure
Traditional medicine
biology
Immunohistochemistry
Liver
Chemistry (miscellaneous)
Malus
030220 oncology & carcinogenesis
Myeloperoxidase
Molecular Medicine
Alkaline phosphatase
Chemical and Drug Induced Liver Injury
hepatotoxicity
medicine.drug_class
CCL4
Protective Agents
digestive system
Article
Anti-inflammatory
lcsh:QD241-441
Superoxide dismutase
03 medical and health sciences
lcsh:Organic chemistry
stomatognathic system
medicine
Animals
Physical and Theoretical Chemistry
030304 developmental biology
Plant Extracts
Organic Chemistry
apple extract
Rats
LC-MS
Disease Models
Animal
chemistry
biology.protein
Carbon tetrachloride
Biomarkers
Zdroj: Molecules
Volume 25
Issue 8
Molecules, Vol 25, Iss 1816, p 1816 (2020)
ISSN: 1420-3049
DOI: 10.3390/molecules25081816
Popis: The aim of this research was to examine the effect of the hydroalcoholic extracts from the peel (APE) and pulp (APP) of a traditional apple cultivar from central Italy (Mela Rosa dei Monti Sibillini) on CCl4-induced hepatotoxicity in rats. Phytoconstituents were determined by liquid chromatography&ndash
mass spectrometry (LC-MS) analysis showing an abundance of proanthocyanidins and flavonol derivatives together with the presence of annurcoic acid in APE. Wistar rats received APE/APP (30 mg/kg oral administration) for three days before CCl4 injection (2 mL/kg intraperitoneal once on the third day). Treatment with both APE and APP prior to CCl4 injection significantly decreased the serum levels of aspartate aminotransferase (AST), alkaline phosphatase (ALP) and alanine aminotransferase (ALT) compared to the CCl4 group. Besides, pretreatment with APE reversed the CCl4 effects on superoxide dismutase (SOD), myeloperoxidase (MPO), tumor necrosis factor-&alpha
(TNF-&alpha
) and interleukin-1beta (IL-1&beta
) levels in liver tissue in rats and reduced tissue damage as shown in hematoxylin and eosin staining. These results showed that this ancient Italian apple is worthy of use in nutraceuticals and dietary supplements to prevent and/or protect against liver disorders.
Databáze: OpenAIRE
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