ELISA versus Conventional Methods of Diagnosing Endemic Brucellosis
| Autor: | Ravindra R Walvekar, Satish K Amarnath, Aisha M Parande, B G Mantur, Rupali S Shinde, Masiyappa Chandrashekar, Giridhar A Patil, Arun S Desai, Satish V. Patil, Mahantesh V. Parande |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Adolescent Endemic Diseases India Enzyme-Linked Immunosorbent Assay Brucella Sensitivity and Specificity Brucellosis Immunoglobulin G Young Adult Agglutination Tests Virology medicine Humans Child Aged Mercaptoethanol Aged 80 and over biology business.industry Infant Articles Elisa assay Middle Aged biology.organism_classification medicine.disease Antibodies Bacterial Agglutination (biology) Infectious Diseases Immunoglobulin M Child Preschool Immunology biology.protein Female Parasitology Endemic diseases Antibody business |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 83:314-318 |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.2010.09-0790 |
| Popis: | The diagnostic value of enzyme-linked immunosorbent assay (ELISA) was evaluated when blood specimens of 92 patients suspected of brucellosis underwent the ELISA (IgM and IgG), standard tube agglutination (SAT), and 2-mercaptoethanol (2-ME) tests and blood cultures; 38 sera from non-brucellosis patients and 34 sera from blood donors were also subjected to ELISA, SAT, and 2-ME tests. SAT was able to pinpoint only 23 (25%), whereas ELISA confirmed the etiology in 56 (60.9%; P < 0.001) patients with brucellosis, including 31 culture-confirmed cases. The sensitivity and specificity of ELISA were 100% and 71.31%, respectively. Because they were confirmed by ELISA, the diagnosis could never be excluded with SAT in 33 cases. ELISA has been found to be more sensitive in acute (28% higher sensitivity; P < 0.02) and chronic (55% higher sensitivity; P < 0.01) cases. For accurate diagnosis in suspected brucellosis cases detection, we recommend both ELISA IgM and IgG tests. ELISA IgG and 2-ME tests seem to be promising tools in judging prognosis. |
| Databáze: | OpenAIRE |
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