| Autor: |
Caitlin Hatton, Nadisha Gamage, Pooja Sridhar, Stephen C.L. Hall, Peter J. Wotherspoon, Gareth W. Hughes, Luke A. Clifton, James Whitehouse, Claire S. Laxton, Timothy J. Knowles, John Wright, Mark Jeeves, David J. Hardy |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Biophys J |
| ISSN: |
0006-3495 |
| Popis: |
The outer membrane of Gram-negative bacteria presents a robust physicochemical barrier protecting the cell from both the natural environment and acting as the first line of defense against antimicrobial materials. The proteins situated within the outer membrane are responsible for a range of biological functions including controlling influx and efflux. These outer membrane proteins (OMPs) are ultimately inserted and folded within the membrane by the β-barrel assembly machine (Bam) complex. The precise mechanism by which the Bam complex folds and inserts OMPs remains unclear. Here, we have developed a platform for investigating Bam-mediated OMP insertion. By derivatizing a gold surface with a copper-chelating self-assembled monolayer, we were able to assemble a planar system containing the complete Bam complex reconstituted within a phospholipid bilayer. Structural characterization of this interfacial protein-tethered bilayer by polarized neutron reflectometry (PNR) revealed distinct regions consistent with known high-resolution models of the Bam complex. Additionally, by monitoring changes of mass associated with OMP insertion by quartz crystal microbalance with dissipation monitoring (QCM-D), we were able to demonstrate the functionality of this system by inserting two diverse OMPs within the membrane, pertactin and OmpT. This platform has promising application in investigating the mechanism of Bam-mediated OMP insertion, in addition to OMP function and activity within a phospholipid bilayer environment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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