Purification and crystallization of human cyclin-dependent kinase 2
| Autor: | Sung-Hou Kim, Hendrik De Bondt, David O. Morgan, Jody Rosenblatt, Jarmila Jancarik |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Protein subunit
Molecular Sequence Data Biology Moths Protein Serine-Threonine Kinases Structural Biology Cyclin-dependent kinase Protein purification CDC2-CDC28 Kinases Animals Humans Protein kinase A Molecular Biology Cells Cultured Base Sequence Kinase Cyclin-dependent kinase 2 Cyclin-Dependent Kinase 2 Cell cycle Cyclin-Dependent Kinases Recombinant Proteins Biochemistry biology.protein Protein crystallization Crystallization Baculoviridae Protein Kinases |
| Zdroj: | Journal of molecular biology. 230(4) |
| ISSN: | 0022-2836 |
| Popis: | The major transitions of the eukaryotic cell cycle are triggered by cyclin-dependent protein kinases. We report the purification and crystallization of the catalytic subunit of human cyclin-dependent kinase 2 (CDK2), which has been implicated in the control of the G1/S transition. CDK2 was purified in large quantities from insect cells infected with a recombinant baculovirus, and crystals of the protein were prepared and subjected to preliminary X-ray diffraction analysis. |
| Databáze: | OpenAIRE |
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