Modeling halotropism: a key role for root tip architecture and reflux loop remodeling in redistributing auxin
| Autor: | van den Berg, Thea, Korver, Ruud A, Testerink, Christa, ten Tusscher, Kirsten, Theoretical Biology and Bioinformatics, Sub Theoretical Biology, Sub Theoretical Biology & Bioinformatics |
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| Přispěvatelé: | Plant Cell Biology (SILS, FNWI), Plant Physiology (SILS, FNWI), Theoretical Biology and Bioinformatics, Sub Theoretical Biology, Sub Theoretical Biology & Bioinformatics |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cellular basis Meristem Arabidopsis Biology Root tip Plant Roots 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Auxin Botany Plant development Molecular Biology Tropism chemistry.chemical_classification Root tropism Indoleacetic Acids Plant roots Arabidopsis Proteins fungi Modeling Auxin transport food and beverages Computer simulation Plants Genetically Modified biology.organism_classification 030104 developmental biology chemistry Biophysics Research Article 010606 plant biology & botany Developmental Biology |
| Zdroj: | Development-The Company of Biologists, 143(18), 3350-3362. Company of Biologists Ltd Development (Cambridge, England), 143(18), 3350. Company of Biologists Ltd Development (Cambridge), 143(18), 3350-3362 Development (Cambridge) 143 (2016) 18 Development (Cambridge, England) |
| ISSN: | 0950-1991 3350-3362 |
| Popis: | A key characteristic of plant development is its plasticity in response to various and dynamically changing environmental conditions. Tropisms contribute to this flexibility by allowing plant organs to grow from or towards environmental cues. Halotropism is a recently described tropism in which plant roots bend away from salt. During halotropism, as in most other tropisms, directional growth is generated through an asymmetric auxin distribution that generates differences in growth rate and hence induces bending. Here, we develop a detailed model of auxin transport in the Arabidopsis root tip and combine this with experiments to investigate the processes generating auxin asymmetry during halotropism. Our model points to the key role of root tip architecture in allowing the decrease in PIN2 at the salt-exposed side of the root to result in a re-routing of auxin to the opposite side. In addition, our model demonstrates how feedback of auxin on the auxin transporter AUX1 amplifies this auxin asymmetry, while a salt-induced transient increase in PIN1 levels increases the speed at which this occurs. Using AUX1-GFP imaging and pin1 mutants, we experimentally confirmed these model predictions, thus expanding our knowledge of the cellular basis of halotropism. Summary: During halotropism, root tip architecture allows for a decrease in PIN2 at the salt-exposed side of the root, resulting in re-routing of auxin to the opposite side; feedback on AUX1 amplifies this auxin asymmetry. |
| Databáze: | OpenAIRE |
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