| Autor: |
H. Hasegawa, John I. Bruce, M.D. Ruff |
| Rok vydání: |
1971 |
| Předmět: |
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| Zdroj: |
Experimental Parasitology. 29:86-93 |
| ISSN: |
0014-4894 |
| DOI: |
10.1016/0014-4894(71)90014-2 |
| Popis: |
The respiration rates of bacteria-free S. mansoni cercariae at 3 and 18 hr after collection were studied using an oxygen monitor. Cercariae at 3 hr after collection utilized more oxygen (152 μl O2/mg total protein/hr) in air-saturated “Hepes” buffer than those in air-saturated “Hepes” buffer containing 0.001 M glucose (52 μl O2/mg total protein/hr). Cercariae at 18 hr consumed 88 μl O2/mg total protein/hr when exogenous glucose (0.001 M) was added to the system. These cercariae were found to be sensitive to decreased oxygen tension since oxygen consumption occurred for only 12–14 min when an exogenous energy source was not provided. After this time a plateau was reached in which oxygen consumption ceased or was so small that it could not be detected. Additional evidence for oxygen tension susceptibility was obtained by the addition of oxygen to the system after the respiration plateau was reached, at which time oxygen consumption resumed until the saturation was reduced to a level comparable with that at which the original plateau was reached. Endogenous glycogen levels were found to correlate with oxygen utilization, as cercariae at 3 hr after collection contained an average of 38.5 ± 3.9 μg glucose per 10 000 cercariae while those at 18 hr contained 1.5 ± 0.7 μg glucose per 10,000 cercariae. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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