Study on the regulation of earthworm physiological function under cadmium stress based on a compound mathematical model
| Autor: | Jiayun Zhuang, Xueli Liu, Mingyuan Xu, Tingxiu Zhang, Haoran Zhou, Dongxing Zhou, Qinghua Shi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Eisenia fetida
Health Toxicology and Mutagenesis Population chemistry.chemical_element 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences Models Biological 03 medical and health sciences chemistry.chemical_compound medicine Animals Soil Pollutants Food science Oligochaeta education Soil Microbiology 030304 developmental biology 0105 earth and related environmental sciences Pharmacology 0303 health sciences Cadmium education.field_of_study biology Microbiota Earthworm General Medicine biology.organism_classification Malondialdehyde Oxidative Stress chemistry Catalase biology.protein Oxidative stress Peroxidase |
| Zdroj: | Environmental toxicology and pharmacology. 80 |
| ISSN: | 1872-7077 |
| Popis: | A cadmium (Cd) stress test was carried out on Eisenia fetida in artificial soil. Six Cd concentration gradient solutions (0, 50, 100, 125, 250 and 500 mg/kg) were prepared. Two treatment groups, short-term stress and long-term stress, were established. The former lasted for 10 days, and the latter lasted for 30 days. The Biolog ECO-microplate culture method was used to determine the utilization of the 31 carbon sources by the microbes in earthworm homogenate. The total protein content (TP), peroxidase activity (POD), catalase activity (CAT), superoxide dismutase activity (SOD), glutathione peroxidase activity (GPX), glutathione-S-transferase activity (GST), malondialdehyde content (MDA) and acetylcholinesterase activity (AChE) in earthworm were determined in order to investigate the regulation of oxidative stress and the functional diversity of microbial communities in earthworms under Cd stress. By combining the entropy weight method (EW) and the technique for order preference by similarity to an ideal solution model (TOPSIS), the physiological functional indices of earthworms were assessed objectively and scientifically, and the physiological changes under the different stress periods were evaluated. The results showed that a Cd-tolerant dominant population appeared in the microbial community under Cd stress. In the short-term test, oxidative stress were more effective in coping with Cd stress than the microbial community, and oxidative stress regulated the microbial community functional diversity. Under long-term Cd stress, the regulatory effect was weak or non-existent. In this study, a new evaluation model was established to explore the regulation process of earthworm on its oxidation stress and the functional diversity of microbial communities under Cd stress, and provide a theoretical basis for revealing the detoxification mechanism of earthworms. |
| Databáze: | OpenAIRE |
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