Quality assurance for genotyping and resistance testing of Clostridium (Clostridioides) difficile isolates - Experiences from the first inter-laboratory ring trial in four German speaking countries
| Autor: | Nathalie Jazmati, Jacqueline Färber, Arthur Pranada, Barbara Gärtner, Gudrun Bettge-Weller, Alexander Schneeberg, Thiên-Trí Lâm, Monique Perrin, Katrin Mantlik, Monika Malecki, Stefan Monecke, Fabian K. Berger, Udo Reischl, Ulrich Nübel, Darius Gawlik, Sven Maurischat, Susanne Kolbe-Busch, Christian Tuschak, Vera Junker, Anja Berger, Maja Adam, Markus Bischoff, Uwe Groß, Lutz von Müller, Christian Seyboldt, Adrian Egli, Andreas Hiergeist, Alexander Mellmann, Stefanie Baumgartner, Colin R. MacKenzie, Felix Pranada |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Genotype Concordance Microbial Sensitivity Tests Microbiology Ribotyping Agar dilution 03 medical and health sciences Internal medicine Drug Resistance Bacterial Medicine Humans Typing Genotyping 030304 developmental biology 0303 health sciences 030306 microbiology business.industry Clostridioides difficile Gold standard (test) 3. Good health Anti-Bacterial Agents Europe Infectious Diseases Clostridium Infections Vancomycin Multilocus sequence typing business medicine.drug Multilocus Sequence Typing |
| Zdroj: | Anaerobe. 61 |
| ISSN: | 1095-8274 |
| Popis: | Clostridium (Clostridioides) difficile is a major cause of nosocomial diarrhoea. A first inter-laboratory ring trial was performed in four European countries to evaluate the genotyping and antibiotic susceptibility testing (AST) accuracy. Six C. difficile isolates representing the epidemiologic important ribotypes (RT), RT001, RT002, RT010, RT014, RT027, and RT078 were blinded and send to 21 participating laboratories. Participants tested the samples with their genotyping and AST methods in use for concordance with reference. A total of 21 genotyping- and 14 antimicrobial susceptibility data sets were obtained. Ribotyping (11 participants) correctly identified most RTs (median 91% concordance rate) except for RT002, which was misidentified in 4/11 reports. However, this isolate was correctly asserted to RT002 after an update of a publicly available ribotyping database. Multilocus sequence typing, surface layer sequence typing, DNA microarray based genotyping, and whole genome sequencing, which were used by 1–3 participants, identified all six isolates correctly. AST was done by epsilometry by the participants and compared to agar dilution data determined by the coordinating reference centre. Susceptibilities against metronidazole, moxifloxacin, and vancomycin were correctly identified in 235 of 237 cases and in accordance to agar dilution as the gold standard. Genotyping of the C. difficile test strains revealed a remarkable high concordance on the level of ribotypes with a wide variety of methods. Epsilometry appears to be a reliable method for AST of C. difficile isolates in routine clinical microbiology laboratories. |
| Databáze: | OpenAIRE |
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