Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic rats may lead to increased seizure susceptibility
| Autor: | Norberto Garcia-Cairasco, Gabriela Machado Parreira, Cristiane Queixa Tilelli, Lívea Dornela Godoy, Eduardo H.L. Umeoka, Israel José Pereira Garcia, Leandro A. Barbosa, Hérica de Lima Santos, Maria Daniela Aparecida Resende, Daniela Bueno Sartori |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Kindling Neurologic/physiology Sodium-Potassium-Exchanging ATPase/metabolism Seizures/etiology medicine.disease_cause Lipid peroxidation chemistry.chemical_compound 0302 clinical medicine Neurons/enzymology Protein Isoforms chemistry.chemical_classification Membrane potential Adenosine Triphosphatases Neurons Prosencephalon/enzymology biology General Neuroscience Glutathione peroxidase Brain Stem/enzymology Brainstem Sodium-Potassium-Exchanging ATPase Protein Isoforms/metabolism Gene isoform medicine.medical_specialty Oxidative Stress/physiology Superoxide dismutase 03 medical and health sciences Prosencephalon Seizures Internal medicine medicine Kindling Neurologic Animals Rats Wistar Molecular Biology Glutathione Peroxidase Glutathione Peroxidase/metabolism RATOS WISTAR Adenosine Triphosphatases/metabolism Rats Disease Models Animal Oxidative Stress 030104 developmental biology Endocrinology Acoustic Stimulation/adverse effects chemistry Acoustic Stimulation Forebrain biology.protein Neurology (clinical) Lipid Peroxidation 030217 neurology & neurosurgery Oxidative stress Developmental Biology Brain Stem |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Parreira, G M, Resende, M D A, Garcia, I J P, Sartori, D B, Umeoka, E H D L, Godoy, L D, Garcia-Cairasco, N, Barbosa, L A, Santos, H D L & Tilelli, C Q 2018, ' Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic rats may lead to increased seizure susceptibility ', Brain Research, vol. 1679, pp. 171-178 . https://doi.org/10.1016/j.brainres.2017.12.001 |
| DOI: | 10.1016/j.brainres.2017.12.001 |
| Popis: | The Wistar Audiogenic Rat (WAR) is a well-characterized seizure-prone, inbred rodent strain that, when acutely stimulated with high-intensity sounds, develops brainstem-dependent tonic-clonic seizures that can evolve to limbic-like, myoclonic (forebrain) seizures when the acoustic stimuli are presented chronically (audiogenic kindling). In order to investigate possible mechanisms underlying WAR susceptibility to seizures, we evaluated Na,K-ATPase activity, Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and oxidative stress markers in whole forebrain and whole brainstem samples of naive WAR, as compared to samples from control Wistar rats. We also evaluated the expression levels of α1 and α3 isoforms of Na,K-ATPase in forebrain samples. We observed increased Na,K-ATPase activity in forebrain samples and increased oxidative stress markers (lipid peroxidation, glutathione peroxidase and superoxide dismutase) in brainstem samples of WAR. The Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and expression levels of α1 and α3 isoforms of Na,K-ATPase were unaltered. In view of previous data showing that the membrane potentials from naive WAR’s neurons are less negative than that from neurons from Wistar rats, we suggest that Na,K-ATPase increased activity might be involved in a compensatory mechanism necessary to maintain WAR’s brains normal activity. Additionally, ongoing oxidative stress in the brainstem could bring Na,K-ATPase activity back to normal levels, which may explain why WAR’s present increased susceptibility to seizures triggered by high-intensity sound stimulation. |
| Databáze: | OpenAIRE |
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