Maintenance of pluripotency in mouse ES cells without Trp53
| Autor: | Satoshi Ohtsuka, Masaki Shigeta, Setsuko Fujii, Satomi Nishikawa-Torikai, Kazuhiro Murakami, Hitoshi Niwa, Mariko Yamane |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Homeobox protein NANOG
Genotype Somatic cell Cellular differentiation Rex1 animal diseases Cell Culture Techniques Embryonic Development Gene Expression Biology Leukemia Inhibitory Factor Article Cell Line Gene Knockout Techniques Mice Gene Order Animals Induced pluripotent stem cell Embryonic Stem Cells Multidisciplinary Chimera Gene Expression Profiling Gene targeting Gene Expression Regulation Developmental Cell Differentiation Aneuploidy Embryonic stem cell Cell biology Protein Transport Karyotyping Gene Targeting Tumor Suppressor Protein p53 Leukemia inhibitory factor |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Tumor suppressor Trp53 works as a guardian of the genome in somatic cells. In mouse embryonic stem (ES) cells, it was reported that Trp53 represses pluripotency-associated transcription factor Nanog to induce differentiation. However, since Trp53-null mice develop to term, Trp53 is dispensable for both the maintenance and differentiation of the pluripotent stem cell population in vivo, suggesting the differential functions of Trp53 in ES cells and embryos. To reveal the basis of this discrepancy, here we established a new line of Trp53-null ES cells by sequential gene targeting and evaluated their ability to differentiate in vitro and in vivo. We found that Trp53-null ES cells had defects in differentiation in vitro as reported previously, whereas they were able to contribute to normal development in chimeric embryos. These data indicated that the requirement of Trp53 for maintaining and executing the ES pluripotency is not absolute. |
| Databáze: | OpenAIRE |
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