Copper staining method for extracting biologically active proteins from native gels
| Autor: | Tsutomu Arakawa, Kenneth H. Aoki, Elizabeth A. Mendiaz, Jette Wypych, Mei Zhang, Keith Langley, Tiansheng Li, John S. Philo, Tom Horan, Moeka Kuwamoto, Yoshiko Kita, Jie Wen |
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| Rok vydání: | 2001 |
| Předmět: |
Gel electrophoresis
Chromatography Staining and Labeling Native gel electrophoresis Organic Chemistry Extraction (chemistry) chemistry.chemical_element Proteins Biological activity General Medicine Applied Microbiology and Biotechnology Biochemistry Copper Analytical Chemistry Staining Electrophoresis chemistry Chromatography Gel Electrophoresis Polyacrylamide Gel Molecular Biology Gels Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 65(6) |
| ISSN: | 0916-8451 |
| Popis: | An attempt was made to make protein bands visible on native gel using copper staining, since such a mild staining procedure would make the entire native gel electrophoresis process non-denaturing. Copper staining not only was able to detect various proteins on native gel with reasonable sensitivity, but also made extraction and recovery of active proteins possible from the gel using a gentle procedure. |
| Databáze: | OpenAIRE |
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