Histologic Distribution, Fragment Cloning, and Sequence Analysis of G Protein Couple Receptor 30 in Rat Submaxillary Gland
Autor: | Lei Chen, Xiao-Peng Mei, Xude Sun, Guihe Zhang, Changjun Gao |
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Rok vydání: | 2011 |
Předmět: |
Male
Cytoplasm medicine.medical_specialty Histology Submandibular Gland Estrogen receptor In situ hybridization Biology Supraoptic nucleus Receptors G-Protein-Coupled Rats Sprague-Dawley Internal medicine medicine Animals RNA Messenger Cloning Molecular Receptor In Situ Hybridization Ecology Evolution Behavior and Systematics Messenger RNA Salivary gland Reverse Transcriptase Polymerase Chain Reaction Cell Membrane Epithelial Cells Sequence Analysis DNA Immunohistochemistry Molecular biology Rats medicine.anatomical_structure Endocrinology Anatomy GPER Duct (anatomy) Biotechnology |
Zdroj: | The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology. 294:706-711 |
ISSN: | 1932-8486 |
DOI: | 10.1002/ar.21349 |
Popis: | Recent studies indicated that G protein couple receptor 30 (GPR30), a nongenomic estrogen receptor, is widely expressed in many organ systems inducing many quick reaction of estrogen. However, there was rare report about the expression of GPR30 in the salivary gland. In the present study, we investigated the distribution of GPR30 in rat submaxillary gland by means of immunohistochemistry and in situ hybridization. GPR30 core sequences were amplified by RT-PCR with total RNA extracted from rat submaxillary gland and were analyzed by sequencing with Sanger's method. The results showed that the epithelial cells of serous alveoli and granular convoluted duct in rat submaxillary gland displayed GPR30-immunoreactivity on the plasma membrane and cytoplasm. Moreover, GPR30 mRNA hybridization signals were also detected in the cytoplasm of the above cells. GPR30 cDNA sequence cloned from rat submaxillary gland is identical to that of GPR30 from rat paraventricular and supraoptic nucleus. In conclusion, the expression of GPR30 in the serous and granular epithelial cells of submaxillary gland indicates that submaxillary gland could also be a target organ rapidly responding to estrogen stimulus, and estrogen may be involved in the functional regulation of submaxillary gland. Anat Rec, 2011. © 2011 Wiley-Liss, Inc. |
Databáze: | OpenAIRE |
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